TY - JOUR
T1 - Novel zwitterionic polyphosphorylcholine monolithic column for hydrophilic interaction chromatography
AU - Jiang, Zhengjin
AU - Reilly, John
AU - Everatt, Brian
AU - Smith, Norman W.
PY - 2009/3/20
Y1 - 2009/3/20
N2 - A novel porous zwitterionic monolith was prepared by thermal co-polymerisation of 2-methacryloyloxyethyl phosphorylcholine (MPC) and ethylene glycol dimethacrylate (EDMA) within 100 mu m I.D. capillaries. Mercury intrusion porosimetry, scanning electron microscopy (SEM), micro-HPLC (mu-HPLC), elemental analysis and zeta-potential analysis were used to evaluate the monolithic structure. No evidence of swelling or shrinking of the monolith in different polarity solvents was observed. A typical hydrophilic liquid chromatography(HILIC) mechanism was observed at high organic solvent content (acetonitrile >60%). The phosphorylcholine(PC) functionality has both a positively charged quaternary ammonium and a negatively charged phosphate group. For charged analytes, a weak electrostatic interaction was also observed by studying the influence of mobile phase pH and salt concentration on their retentions on the poly(MPC-co-EDMA) monolithic column. The optimised poly(MPC-co-EDMA) monolith showed very good selectivities for a range of polar test analytes, especially small peptides. This might be ascribed to the good biocompatibility of PC functionality. At low organic solvent content, baseline separation was also observed for a test mixture of seven alkylphenones by a reversed-phase separation mechanism. (c) 2009 Elsevier B.V. All rights reserved.
AB - A novel porous zwitterionic monolith was prepared by thermal co-polymerisation of 2-methacryloyloxyethyl phosphorylcholine (MPC) and ethylene glycol dimethacrylate (EDMA) within 100 mu m I.D. capillaries. Mercury intrusion porosimetry, scanning electron microscopy (SEM), micro-HPLC (mu-HPLC), elemental analysis and zeta-potential analysis were used to evaluate the monolithic structure. No evidence of swelling or shrinking of the monolith in different polarity solvents was observed. A typical hydrophilic liquid chromatography(HILIC) mechanism was observed at high organic solvent content (acetonitrile >60%). The phosphorylcholine(PC) functionality has both a positively charged quaternary ammonium and a negatively charged phosphate group. For charged analytes, a weak electrostatic interaction was also observed by studying the influence of mobile phase pH and salt concentration on their retentions on the poly(MPC-co-EDMA) monolithic column. The optimised poly(MPC-co-EDMA) monolith showed very good selectivities for a range of polar test analytes, especially small peptides. This might be ascribed to the good biocompatibility of PC functionality. At low organic solvent content, baseline separation was also observed for a test mixture of seven alkylphenones by a reversed-phase separation mechanism. (c) 2009 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.chroma.2009.01.028
DO - 10.1016/j.chroma.2009.01.028
M3 - Article
VL - 1216
SP - 2439
EP - 2448
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 12
ER -