Abstract
A biochemical explanation of development from the
fertilized egg to the adult requires an understanding
of the proteins and RNAs expressed over time during
embryogenesis. We present a comprehensive characterization
of protein and mRNA dynamics across
early development in Xenopus. Surprisingly, we find
that most protein levels change little and duplicated
genes are expressed similarly. While the correlation
between protein and mRNA levels is poor, a mass
action kinetics model parameterized using protein
synthesis and degradation rates regresses protein
dynamics to RNA dynamics, corrected for initial protein
concentration. This study provides detailed data
for absolute levels of 10,000 proteins and 28,000
transcripts via a convenient web portal, a rich
resource for developmental biologists. It underscores
the lasting impact of maternal dowry, finds
surprisingly few cases where degradation alone
drives a change in protein level, and highlights the
importance of transcription in shaping the dynamics
of the embryonic proteome.
fertilized egg to the adult requires an understanding
of the proteins and RNAs expressed over time during
embryogenesis. We present a comprehensive characterization
of protein and mRNA dynamics across
early development in Xenopus. Surprisingly, we find
that most protein levels change little and duplicated
genes are expressed similarly. While the correlation
between protein and mRNA levels is poor, a mass
action kinetics model parameterized using protein
synthesis and degradation rates regresses protein
dynamics to RNA dynamics, corrected for initial protein
concentration. This study provides detailed data
for absolute levels of 10,000 proteins and 28,000
transcripts via a convenient web portal, a rich
resource for developmental biologists. It underscores
the lasting impact of maternal dowry, finds
surprisingly few cases where degradation alone
drives a change in protein level, and highlights the
importance of transcription in shaping the dynamics
of the embryonic proteome.
| Original language | English |
|---|---|
| Pages (from-to) | 383 |
| Number of pages | 394 |
| Journal | Developmental Cell |
| Volume | 35 |
| Publication status | Published - 9 Nov 2015 |