TY - JOUR
T1 - Probing the Mobilome
T2 - Discoveries in the Dynamic Microbiome
AU - Moyes, Dave
AU - Carr, Vicky
AU - Mullany, peter
AU - Hill, Colin
AU - Shkoporov, Andrey
PY - 2020/5/10
Y1 - 2020/5/10
N2 - There has been an explosion of metagenomic data representing human, animal,and environmental microbiomes. This provides an unprecedented opportunityfor comparative and longitudinal studies of many functional aspects of themicrobiome that go beyond taxonomic classification, such as profiling geneticdeterminants of antimicrobial resistance, interactions with the host, potentiallyclinically relevant functions, and the role of mobile genetic elements (MGEs).One of the most important but least studied of these aspects are the MGEs, col-lectively referred to as the 'mobilome'. Here we elaborate on the benefits and lim-itations of using different metagenomic protocols, discuss the relative merits ofvarious sequencing technologies, and highlight relevant bioinformatics toolsand pipelines to predict the presence of MGEs and their microbial hosts.
AB - There has been an explosion of metagenomic data representing human, animal,and environmental microbiomes. This provides an unprecedented opportunityfor comparative and longitudinal studies of many functional aspects of themicrobiome that go beyond taxonomic classification, such as profiling geneticdeterminants of antimicrobial resistance, interactions with the host, potentiallyclinically relevant functions, and the role of mobile genetic elements (MGEs).One of the most important but least studied of these aspects are the MGEs, col-lectively referred to as the 'mobilome'. Here we elaborate on the benefits and lim-itations of using different metagenomic protocols, discuss the relative merits ofvarious sequencing technologies, and highlight relevant bioinformatics toolsand pipelines to predict the presence of MGEs and their microbial hosts.
UR - http://www.scopus.com/inward/record.url?scp=85085031533&partnerID=8YFLogxK
U2 - 10.1016/j.tim.2020.05.003
DO - 10.1016/j.tim.2020.05.003
M3 - Review article
SN - 0966-842X
JO - Trends in Microbiology
JF - Trends in Microbiology
ER -
