TY - JOUR
T1 - Production of L-phenylacetyl carbinol by biotransformation
T2 - product and by-product formation and activities of the key enzymes in wild-type and ADH isoenzyme mutants of Saccharomyces cerevisiae
AU - Nikolova, P
AU - Ward, O P
PY - 1991/8/20
Y1 - 1991/8/20
N2 - The capacities of yeast wild-type and mutants strains known to lack specific ADH isoenzymes to produce L-phenylacetyl carbinol (PAC) and benzyl alcohol in biotransformation trials were also investigated. Pyruvate decarboxylase activity, responsible for PAC formation and ADH activity, which can participate in reduction of benzaldehyde to benzyl alcohol, was also determined in each strain. In addition, the capacity of each strain to produce ethanol was investigated. Mutant strains lacking all of the isoenzymes, ADH-I, ADH-II, and ADH-III, still exhibited some ADH activity and were capable of production of benzyl alcohol and ethanol.
AB - The capacities of yeast wild-type and mutants strains known to lack specific ADH isoenzymes to produce L-phenylacetyl carbinol (PAC) and benzyl alcohol in biotransformation trials were also investigated. Pyruvate decarboxylase activity, responsible for PAC formation and ADH activity, which can participate in reduction of benzaldehyde to benzyl alcohol, was also determined in each strain. In addition, the capacity of each strain to produce ethanol was investigated. Mutant strains lacking all of the isoenzymes, ADH-I, ADH-II, and ADH-III, still exhibited some ADH activity and were capable of production of benzyl alcohol and ethanol.
U2 - 10.1002/bit.260380507
DO - 10.1002/bit.260380507
M3 - Article
C2 - 18604807
SN - 0006-3592
VL - 38
SP - 493
EP - 498
JO - BIOTECHNOLOGY AND BIOENGINEERING
JF - BIOTECHNOLOGY AND BIOENGINEERING
IS - 5
M1 - N/A
ER -