Psoriatic and rheumatoid arthritis joints differ in the composition of CD8+ tissue-resident memory T cell subsets

Giovanni A.M. Povoleri; Lucy E. Durham; Elizabeth H. Gray; Sylvine Lalnunhlimi; Shichina Kannambath; Michael J. Pitcher; Pawan Dhami; Thomas Leeuw; Sarah E. Ryan; Kathryn J.A. Steel; Bruce W. Kirkham; Leonie S. Taams

doi:10.1016/j.celrep.2023.112514

Psoriatic and rheumatoid arthritis joints differ in the composition of CD8+ tissue-resident memory T cell subsets

Giovanni A.M. Povoleri, Lucy E. Durham, Elizabeth H. Gray, Sylvine Lalnunhlimi, Shichina Kannambath, Michael J. Pitcher, Pawan Dhami, Thomas Leeuw, Sarah E. Ryan, Kathryn J.A. Steel, Bruce W. Kirkham, Leonie S. Taams^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

CD69+CD103+ tissue-resident memory T (T_RM) cells are important drivers of inflammation. To decipher their role in inflammatory arthritis, we apply single-cell, high-dimensional profiling to T cells from the joints of patients with psoriatic arthritis (PsA) or rheumatoid arthritis (RA). We identify three groups of synovial CD8+CD69+CD103+ T_RM cells: cytotoxic and regulatory T (Treg)-like T_RM cells are present in both PsA and RA, while CD161+CCR6+ type 17-like T_RM cells with a pro-inflammatory cytokine profile (IL-17A+TNFα+IFNγ+) are specifically enriched in PsA. In contrast, only one population of CD4+CD69+CD103+ T_RM cells is detected and at similarly low frequencies in both diseases. Type 17-like CD8+ T_RM cells have a distinct transcriptomic signature and a polyclonal, but distinct, TCR repertoire. Type 17-like cells are also enriched in CD8+CD103− T cells in PsA compared with RA. These findings illustrate differences in the immunopathology of PsA and RA, with a particular enrichment for type 17 CD8+ T cells in the PsA joint.

Original language	English
Article number	112514
Journal	Cell Reports
Volume	42
Issue number	5
DOIs	https://doi.org/10.1016/j.celrep.2023.112514
Publication status	Published - 30 May 2023

Keywords

CD103
CD8+ T cells
CP: Immunology
CyTOF
joint
psoriatic arthritis
rheumatoid arthritis
scRNA-seq
synovial fluid
T
Tc17 cells

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10.1016/j.celrep.2023.112514

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Povoleri, G. A. M., Durham, L. E., Gray, E. H., Lalnunhlimi, S., Kannambath, S., Pitcher, M. J., Dhami, P., Leeuw, T., Ryan, S. E., Steel, K. J. A., Kirkham, B. W., & Taams, L. S. (2023). Psoriatic and rheumatoid arthritis joints differ in the composition of CD8+ tissue-resident memory T cell subsets. Cell Reports, 42(5), Article 112514. https://doi.org/10.1016/j.celrep.2023.112514

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title = "Psoriatic and rheumatoid arthritis joints differ in the composition of CD8+ tissue-resident memory T cell subsets",

abstract = "CD69+CD103+ tissue-resident memory T (TRM) cells are important drivers of inflammation. To decipher their role in inflammatory arthritis, we apply single-cell, high-dimensional profiling to T cells from the joints of patients with psoriatic arthritis (PsA) or rheumatoid arthritis (RA). We identify three groups of synovial CD8+CD69+CD103+ TRM cells: cytotoxic and regulatory T (Treg)-like TRM cells are present in both PsA and RA, while CD161+CCR6+ type 17-like TRM cells with a pro-inflammatory cytokine profile (IL-17A+TNFα+IFNγ+) are specifically enriched in PsA. In contrast, only one population of CD4+CD69+CD103+ TRM cells is detected and at similarly low frequencies in both diseases. Type 17-like CD8+ TRM cells have a distinct transcriptomic signature and a polyclonal, but distinct, TCR repertoire. Type 17-like cells are also enriched in CD8+CD103− T cells in PsA compared with RA. These findings illustrate differences in the immunopathology of PsA and RA, with a particular enrichment for type 17 CD8+ T cells in the PsA joint.",

keywords = "CD103, CD8+ T cells, CP: Immunology, CyTOF, joint, psoriatic arthritis, rheumatoid arthritis, scRNA-seq, synovial fluid, T, Tc17 cells",

author = "Povoleri, {Giovanni A.M.} and Durham, {Lucy E.} and Gray, {Elizabeth H.} and Sylvine Lalnunhlimi and Shichina Kannambath and Pitcher, {Michael J.} and Pawan Dhami and Thomas Leeuw and Ryan, {Sarah E.} and Steel, {Kathryn J.A.} and Kirkham, {Bruce W.} and Taams, {Leonie S.}",

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AU - Povoleri, Giovanni A.M.

AU - Durham, Lucy E.

AU - Gray, Elizabeth H.

AU - Lalnunhlimi, Sylvine

AU - Kannambath, Shichina

AU - Pitcher, Michael J.

AU - Dhami, Pawan

AU - Leeuw, Thomas

AU - Ryan, Sarah E.

AU - Steel, Kathryn J.A.

AU - Kirkham, Bruce W.

AU - Taams, Leonie S.

PY - 2023/5/30

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AB - CD69+CD103+ tissue-resident memory T (TRM) cells are important drivers of inflammation. To decipher their role in inflammatory arthritis, we apply single-cell, high-dimensional profiling to T cells from the joints of patients with psoriatic arthritis (PsA) or rheumatoid arthritis (RA). We identify three groups of synovial CD8+CD69+CD103+ TRM cells: cytotoxic and regulatory T (Treg)-like TRM cells are present in both PsA and RA, while CD161+CCR6+ type 17-like TRM cells with a pro-inflammatory cytokine profile (IL-17A+TNFα+IFNγ+) are specifically enriched in PsA. In contrast, only one population of CD4+CD69+CD103+ TRM cells is detected and at similarly low frequencies in both diseases. Type 17-like CD8+ TRM cells have a distinct transcriptomic signature and a polyclonal, but distinct, TCR repertoire. Type 17-like cells are also enriched in CD8+CD103− T cells in PsA compared with RA. These findings illustrate differences in the immunopathology of PsA and RA, with a particular enrichment for type 17 CD8+ T cells in the PsA joint.

KW - CD103

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KW - CP: Immunology

KW - CyTOF

KW - joint

KW - psoriatic arthritis

KW - rheumatoid arthritis

KW - scRNA-seq

KW - synovial fluid

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ER -

Psoriatic and rheumatoid arthritis joints differ in the composition of CD8+ tissue-resident memory T cell subsets

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