Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

Riana Gaifulina; Daren J. Caruana; Dahmane Oukrif; Naomi J. Guppy; Siân Culley; Robert Brown; Ian Bell; Manuel Rodriguez-Justo; Katherine Lau; Geraint M.H. Thomas

doi:10.1039/c9an01030k

Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

Riana Gaifulina, Daren J. Caruana, Dahmane Oukrif, Naomi J. Guppy, Siân Culley, Robert Brown, Ian Bell, Manuel Rodriguez-Justo, Katherine Lau^*, Geraint M.H. Thomas

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing.

Original language	English
Pages (from-to)	1499-1510
Number of pages	12
Journal	Analyst
Volume	145
Issue number	4
DOIs	https://doi.org/10.1039/c9an01030k
Publication status	Published - 21 Feb 2020

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title = "Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis",

abstract = "Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing.",

author = "Riana Gaifulina and Caruana, {Daren J.} and Dahmane Oukrif and Guppy, {Naomi J.} and Si{\^a}n Culley and Robert Brown and Ian Bell and Manuel Rodriguez-Justo and Katherine Lau and Thomas, {Geraint M.H.}",

note = "Funding Information: This study was funded through the UCL Impact PhD Scheme (to RG) with support from Renishaw Spectroscopy Division (Renishaw plc) and the Medical Research Council (to GT, reference MC_PC_14118 v.2 PO 050471026). Dr Manuel Rodriguez-Justo is supported by the UCLH/UCL NIHR Biomedical Research Centre. Dr Si{\^a}n Culley is funded by the Wellcome Trust. We would like to thank UCLH Advanced Diagnostics for providing help with tissue processing. We would also like to extend our gratitude to Mr Tim Smith, Dr Tim Batten and Dr Yan Wong at Renishaw for instrument support. Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

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doi = "10.1039/c9an01030k",

language = "English",

volume = "145",

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T1 - Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

AU - Gaifulina, Riana

AU - Caruana, Daren J.

AU - Oukrif, Dahmane

AU - Guppy, Naomi J.

AU - Culley, Siân

AU - Brown, Robert

AU - Bell, Ian

AU - Rodriguez-Justo, Manuel

AU - Lau, Katherine

AU - Thomas, Geraint M.H.

N1 - Funding Information: This study was funded through the UCL Impact PhD Scheme (to RG) with support from Renishaw Spectroscopy Division (Renishaw plc) and the Medical Research Council (to GT, reference MC_PC_14118 v.2 PO 050471026). Dr Manuel Rodriguez-Justo is supported by the UCLH/UCL NIHR Biomedical Research Centre. Dr Siân Culley is funded by the Wellcome Trust. We would like to thank UCLH Advanced Diagnostics for providing help with tissue processing. We would also like to extend our gratitude to Mr Tim Smith, Dr Tim Batten and Dr Yan Wong at Renishaw for instrument support. Publisher Copyright: © 2020 The Royal Society of Chemistry.

PY - 2020/2/21

Y1 - 2020/2/21

N2 - Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing.

AB - Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing.

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SN - 0003-2654

VL - 145

SP - 1499

EP - 1510

JO - Analyst

JF - Analyst

IS - 4

ER -

Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

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