TY - CHAP
T1 - Salivary Gland Development in Culture
AU - Gaete, Marcia
AU - Teshima, Tathyane H.N.
AU - Chatzeli, Lemonia
AU - Tucker, Abigail S.
N1 - Funding Information:
MG is funded by grant Redes Internacionales para Investigadores en Etapa Inicial, REDI170595, ANID (ex-CONICYT); THNT (Clinical Lecturer, CL-2019-18-009) is funded by Health Education England (HEE) / National Institute for Health Research (NIHR) for this research project. The views expressed in this publication are those of the authors and not necessarily those of the NIHR, NHS or the UK Department of Health and Social Care. LC is funded by the Herchel Smith Fund. We thank to Constanza Daza for editing the scheme designed by THNT.
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Salivary glands are branching organs which develop by bud and cleft formation to create an organ with a large surface area. The epithelium and mesenchyme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture, allowing access to the tissue to follow development and manipulate the tissue interactions, and signals. To culture glands, the filter-grid method has been widely used, allowing the development of salivary glands cultured as a whole organ, or the gland epithelium in isolation, or with the surrounding craniofacial tissue in a cranial slice. Here, we describe the methods for each approach and show the applicability of culturing glands from a wide variety of species: mouse, snake, and human. The resulting samples and data from these cultures can be employed for morphological and molecular analysis, with some examples described in this chapter, bringing valuable knowledge to our understanding of branching morphogenesis.
AB - Salivary glands are branching organs which develop by bud and cleft formation to create an organ with a large surface area. The epithelium and mesenchyme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture, allowing access to the tissue to follow development and manipulate the tissue interactions, and signals. To culture glands, the filter-grid method has been widely used, allowing the development of salivary glands cultured as a whole organ, or the gland epithelium in isolation, or with the surrounding craniofacial tissue in a cranial slice. Here, we describe the methods for each approach and show the applicability of culturing glands from a wide variety of species: mouse, snake, and human. The resulting samples and data from these cultures can be employed for morphological and molecular analysis, with some examples described in this chapter, bringing valuable knowledge to our understanding of branching morphogenesis.
KW - Branching morphogenesis
KW - Clefting
KW - Human organ culture
KW - Salivary gland
KW - Snake organ culture
UR - http://www.scopus.com/inward/record.url?scp=85121729633&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-1847-9_19
DO - 10.1007/978-1-0716-1847-9_19
M3 - Chapter
C2 - 34913130
AN - SCOPUS:85121729633
T3 - Methods in Molecular Biology
SP - 277
EP - 294
BT - Methods in Molecular Biology
PB - Humana Press Inc
ER -