Silica passivated conjugated polymer nanoparticles for biological imaging applications

Struan Bourke; Laura Urbano; Antoni Olona; Ferran Valderrama; Lea Ann Dailey; Mark A. Green

doi:10.1117/12.2252035

Silica passivated conjugated polymer nanoparticles for biological imaging applications

Struan Bourke, Laura Urbano, Antoni Olona, Ferran Valderrama, Lea Ann Dailey, Mark A. Green

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution › peer-review

2 Citations (Scopus)

180 Downloads (Pure)

Abstract

Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

Original language	English
Title of host publication	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX
Publisher	SPIE
Volume	10079
ISBN (Electronic)	9781510605992
DOIs	https://doi.org/10.1117/12.2252035
Publication status	Published - 21 Feb 2017
Event	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX 2017 - San Francisco, United States Duration: 30 Jan 2017 → 31 Jan 2017

Conference

Conference	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX 2017
Country/Territory	United States
City	San Francisco
Period	30/01/2017 → 31/01/2017

Keywords

Biological imaging
Cancer imaging
Conjugated polymers
Nanoparticles
Silica shells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.2252035

Silica passivated conjugated polymer_BOURKE_Published21February2017_GREEN AAMAccepted author manuscript, 891 KB

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abstract = "Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.",

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Bourke, S , Urbano, L, Olona, A, Valderrama, F, Ann Dailey, L & Green, MA 2017, Silica passivated conjugated polymer nanoparticles for biological imaging applications. in Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX. vol. 10079, 100790A, SPIE, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX 2017, San Francisco, United States, 30/01/2017. https://doi.org/10.1117/12.2252035

Silica passivated conjugated polymer nanoparticles for biological imaging applications. / Bourke, Struan ; Urbano, Laura; Olona, Antoni et al.
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX. Vol. 10079 SPIE, 2017. 100790A.

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution › peer-review

TY - CHAP

T1 - Silica passivated conjugated polymer nanoparticles for biological imaging applications

AU - Bourke, Struan

AU - Urbano, Laura

AU - Olona, Antoni

AU - Valderrama, Ferran

AU - Ann Dailey, Lea

AU - Green, Mark A.

PY - 2017/2/21

Y1 - 2017/2/21

N2 - Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

AB - Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However, as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new fluorescent probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, bright, photo-stable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell. By incubating the CN-PPV silica shelled cross-linked (SSCL) nanoparticles in mammalian (HeLa) cells; we were able to show that cellular uptake occurred. Uptake was also shown by incubating the nanoparticles in RWPE1, WPE1-NB26 and WPE1- NA22 prostate cancer cell lines. Finally, HEK cells were used to show the particles had limited cytotoxicity.

KW - Biological imaging

KW - Cancer imaging

KW - Conjugated polymers

KW - Nanoparticles

KW - Silica shells

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DO - 10.1117/12.2252035

M3 - Other chapter contribution

AN - SCOPUS:85020263652

VL - 10079

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX

PB - SPIE

T2 - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX 2017

Y2 - 30 January 2017 through 31 January 2017

ER -

Silica passivated conjugated polymer nanoparticles for biological imaging applications

Abstract

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Keywords

UN SDGs

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