Simultaneous Measurement of Ionic Current and Fluorescence from Single Protein Pores

Andrew J. Heron; James R. Thompson; Bríd Cronin; Hagan Bayley; Mark I. Wallace

doi:10.1021/ja808128s

Simultaneous Measurement of Ionic Current and Fluorescence from Single Protein Pores

Andrew J. Heron, James R. Thompson, Bríd Cronin, Hagan Bayley, Mark I. Wallace

Chemistry

Research output: Contribution to journal › Article › peer-review

111 Citations (Scopus)

Abstract

The ability to simultaneously monitor both the ionic current and fluorescence from membrane channels and pores has the potential to link structural changes with function in such proteins. We present a new method for simultaneously measuring single-channel electrical currents and fluorescence from membrane proteins by using water-in-oil droplet bilayers. We demonstrate the simultaneous fluorescence and electrical detection of stochastic blocking by cyclodextrin in multiple staphylococcal alpha-hemolysin pores. The combined fluorescence signal from individual pores exhibits the same sequence of blocking events as the total current recording, showing that the two signals from each pore are correlated.

Original language	English
Pages (from-to)	1652-1653
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	131
Issue number	5
DOIs	https://doi.org/10.1021/ja808128s
Publication status	Published - 1 Feb 2009

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https://doi.org/10.1021%2Fja808128s

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abstract = "The ability to simultaneously monitor both the ionic current and fluorescence from membrane channels and pores has the potential to link structural changes with function in such proteins. We present a new method for simultaneously measuring single-channel electrical currents and fluorescence from membrane proteins by using water-in-oil droplet bilayers. We demonstrate the simultaneous fluorescence and electrical detection of stochastic blocking by cyclodextrin in multiple staphylococcal alpha-hemolysin pores. The combined fluorescence signal from individual pores exhibits the same sequence of blocking events as the total current recording, showing that the two signals from each pore are correlated.",

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AU - Heron, Andrew J.

AU - Thompson, James R.

AU - Cronin, Bríd

AU - Bayley, Hagan

AU - Wallace, Mark I.

PY - 2009/2/1

Y1 - 2009/2/1

N2 - The ability to simultaneously monitor both the ionic current and fluorescence from membrane channels and pores has the potential to link structural changes with function in such proteins. We present a new method for simultaneously measuring single-channel electrical currents and fluorescence from membrane proteins by using water-in-oil droplet bilayers. We demonstrate the simultaneous fluorescence and electrical detection of stochastic blocking by cyclodextrin in multiple staphylococcal alpha-hemolysin pores. The combined fluorescence signal from individual pores exhibits the same sequence of blocking events as the total current recording, showing that the two signals from each pore are correlated.

AB - The ability to simultaneously monitor both the ionic current and fluorescence from membrane channels and pores has the potential to link structural changes with function in such proteins. We present a new method for simultaneously measuring single-channel electrical currents and fluorescence from membrane proteins by using water-in-oil droplet bilayers. We demonstrate the simultaneous fluorescence and electrical detection of stochastic blocking by cyclodextrin in multiple staphylococcal alpha-hemolysin pores. The combined fluorescence signal from individual pores exhibits the same sequence of blocking events as the total current recording, showing that the two signals from each pore are correlated.

U2 - 10.1021/ja808128s

DO - 10.1021/ja808128s

M3 - Article

SN - 0002-7863

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SP - 1652

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 5

ER -

Simultaneous Measurement of Ionic Current and Fluorescence from Single Protein Pores

Abstract

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