siRNA Design and Delivery Based on Carbon Nanotubes

Danyang Li; Khuloud T. Al-Jamal

doi:10.1007/978-1-0716-1298-9_12

siRNA Design and Delivery Based on Carbon Nanotubes

Danyang Li, Khuloud T. Al-Jamal

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Owing to the unique physical and chemical properties of carbon nanotubes, they have been widely explored as delivery vectors for proteins, and nucleic acid etc. after functionalization. Particularly, the modification of carbon nanotubes suited for the delivery of siRNA has been intensely studied over the past decade. The assay described in this chapter allows for realizable quantification of siRNA binding on carbon nanotube-based materials using gel electrophoresis and silencing by flow cytometry when the siRNA complexes are delivered in vitro.

Original language	English
Pages (from-to)	181-193
Number of pages	13
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2282
DOIs	https://doi.org/10.1007/978-1-0716-1298-9_12
Publication status	Published - 2021

Keywords

Binding capacity
Carbon nanotubes
Cationic polymer
N/P ratio
Silencing efficiency
siRNA delivery

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10.1007/978-1-0716-1298-9_12

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KW - Cationic polymer

KW - N/P ratio

KW - Silencing efficiency

KW - siRNA delivery

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siRNA Design and Delivery Based on Carbon Nanotubes

Abstract

Keywords

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