TY - JOUR
T1 - Strategies and outcomes of the first 100 cycles of preimplantation genetic diagnosis at the Guy's and St. Thomas' Center
AU - Pickering, S
AU - Polidoropoulos, N
AU - Caller, J
AU - Scriven, P
AU - Mackie Ogilvie, C
AU - Braude, P
AU - PGD Study Group
AU - Flinter, F
AU - H, Bickerstaff
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Objective: To establish strategies for the implementation of a successful preimplantation genetic diagnosis (PGD) service. Design: Retrospective review of data from a single center. Setting: A United Kingdom National Health Service hospital. Patient(s): Patients (60 couples) were referred for PGD from UK genetic centers. Intervention(s): We followed the protocol of ovarian stimulation, oocyte retrieval, fertilization, single cell biopsy on day 3, and embryo transfer on day 4. Main Outcome Measure(s): Pregnancies unaffected by the familial genetic condition. Result(s): A total of 60 couples was treated for 20 different conditions. Early cycles using nonsequential embryo culture media were less successful (13% pregnancy rate/embryo transfer) than later cycles using sequential media (33.5%). Ninety-four percent of embryos (n = 473) had a single cell removed at biopsy. The overall pregnancy rate was 24% per cycle started, 29% per egg collection, 38% per transfer, and 40% per couple treated. In one cycle, an affected pregnancy followed PGD for spinal muscular atrophy (SMA). Conclusion(s): The use of sequential media and single cell biopsy results in a successful PGD program with encouraging pregnancy rates.
AB - Objective: To establish strategies for the implementation of a successful preimplantation genetic diagnosis (PGD) service. Design: Retrospective review of data from a single center. Setting: A United Kingdom National Health Service hospital. Patient(s): Patients (60 couples) were referred for PGD from UK genetic centers. Intervention(s): We followed the protocol of ovarian stimulation, oocyte retrieval, fertilization, single cell biopsy on day 3, and embryo transfer on day 4. Main Outcome Measure(s): Pregnancies unaffected by the familial genetic condition. Result(s): A total of 60 couples was treated for 20 different conditions. Early cycles using nonsequential embryo culture media were less successful (13% pregnancy rate/embryo transfer) than later cycles using sequential media (33.5%). Ninety-four percent of embryos (n = 473) had a single cell removed at biopsy. The overall pregnancy rate was 24% per cycle started, 29% per egg collection, 38% per transfer, and 40% per couple treated. In one cycle, an affected pregnancy followed PGD for spinal muscular atrophy (SMA). Conclusion(s): The use of sequential media and single cell biopsy results in a successful PGD program with encouraging pregnancy rates.
U2 - 10.1016/S0015-0282(02)04540-5
DO - 10.1016/S0015-0282(02)04540-5
M3 - Article
VL - 79
SP - 81
EP - 90
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 1
ER -