Superresolution by image scanning microscopy using pixel reassignment

Colin J. R. Sheppard; Shalin B. Mehta; Rainer Heintzmann

doi:10.1364/OL.38.002889

Superresolution by image scanning microscopy using pixel reassignment

Colin J. R. Sheppard^*, Shalin B. Mehta, Rainer Heintzmann

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

195 Citations (Scopus)

Abstract

The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.

Original language	English
Article number	N/A
Pages (from-to)	2889-2892
Number of pages	4
Journal	Optics Letters
Volume	38
Issue number	15
DOIs	https://doi.org/10.1364/OL.38.002889
Publication status	Published - 1 Aug 2013

Keywords

STRUCTURED ILLUMINATION MICROSCOPY
CONFOCAL MICROSCOPY
SUPER-RESOLUTION

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10.1364/OL.38.002889

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title = "Superresolution by image scanning microscopy using pixel reassignment",

abstract = "The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.",

keywords = "STRUCTURED ILLUMINATION MICROSCOPY, CONFOCAL MICROSCOPY, SUPER-RESOLUTION",

author = "Sheppard, {Colin J. R.} and Mehta, {Shalin B.} and Rainer Heintzmann",

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AU - Sheppard, Colin J. R.

AU - Mehta, Shalin B.

AU - Heintzmann, Rainer

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N2 - The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.

AB - The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.

KW - STRUCTURED ILLUMINATION MICROSCOPY

KW - CONFOCAL MICROSCOPY

KW - SUPER-RESOLUTION

U2 - 10.1364/OL.38.002889

DO - 10.1364/OL.38.002889

M3 - Article

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SP - 2889

EP - 2892

JO - Optics Letters

JF - Optics Letters

IS - 15

M1 - N/A

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Superresolution by image scanning microscopy using pixel reassignment

Abstract

Keywords

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