The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair

Alexander J. Garvin; Ruth M. Densham; Sarah A. Blair-Reid; Kenny M. Pratt; Helen R. Stone; Daniel Weekes; Kirsty J. Lawrence; Joanna R. Morris

doi:10.1038/embor.2013.141

The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair

Alexander J. Garvin, Ruth M. Densham, Sarah A. Blair-Reid, Kenny M. Pratt, Helen R. Stone, Daniel Weekes, Kirsty J. Lawrence, Joanna R. Morris^*

^*Corresponding author for this work

King's College London

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

SUMO conjugation is known to occur in response to double-stranded DNA breaks in mammalian cells, but whether SUMO deconjugation has a role remains unclear. Here, we show that the SUMO/Sentrin/Smt3-specific peptidase, SENP7, interacts with the chromatin repressive KRAB-associated protein 1 (KAP1) through heterochromatin protein 1 alpha (HP1α). SENP7 promotes the removal of SUMO2/3 from KAP1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. Consequently, in the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. Thus, deSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair.

Original language	English
Pages (from-to)	975-983
Number of pages	9
Journal	EMBO Reports
Volume	14
Issue number	11
DOIs	https://doi.org/10.1038/embor.2013.141
Publication status	Published - 1 Nov 2013

Keywords

homologous recombination
HP1a
KAP1
SENP7
SUMO

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10.1038/embor.2013.141

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title = "The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair",

abstract = "SUMO conjugation is known to occur in response to double-stranded DNA breaks in mammalian cells, but whether SUMO deconjugation has a role remains unclear. Here, we show that the SUMO/Sentrin/Smt3-specific peptidase, SENP7, interacts with the chromatin repressive KRAB-associated protein 1 (KAP1) through heterochromatin protein 1 alpha (HP1α). SENP7 promotes the removal of SUMO2/3 from KAP1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. Consequently, in the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. Thus, deSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair.",

keywords = "homologous recombination, HP1a, KAP1, SENP7, SUMO",

author = "Garvin, {Alexander J.} and Densham, {Ruth M.} and Blair-Reid, {Sarah A.} and Pratt, {Kenny M.} and Stone, {Helen R.} and Daniel Weekes and Lawrence, {Kirsty J.} and Morris, {Joanna R.}",

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year = "2013",

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doi = "10.1038/embor.2013.141",

language = "English",

volume = "14",

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journal = "EMBO Reports",

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T1 - The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair

AU - Garvin, Alexander J.

AU - Densham, Ruth M.

AU - Blair-Reid, Sarah A.

AU - Pratt, Kenny M.

AU - Stone, Helen R.

AU - Weekes, Daniel

AU - Lawrence, Kirsty J.

AU - Morris, Joanna R.

PY - 2013/11/1

Y1 - 2013/11/1

N2 - SUMO conjugation is known to occur in response to double-stranded DNA breaks in mammalian cells, but whether SUMO deconjugation has a role remains unclear. Here, we show that the SUMO/Sentrin/Smt3-specific peptidase, SENP7, interacts with the chromatin repressive KRAB-associated protein 1 (KAP1) through heterochromatin protein 1 alpha (HP1α). SENP7 promotes the removal of SUMO2/3 from KAP1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. Consequently, in the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. Thus, deSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair.

AB - SUMO conjugation is known to occur in response to double-stranded DNA breaks in mammalian cells, but whether SUMO deconjugation has a role remains unclear. Here, we show that the SUMO/Sentrin/Smt3-specific peptidase, SENP7, interacts with the chromatin repressive KRAB-associated protein 1 (KAP1) through heterochromatin protein 1 alpha (HP1α). SENP7 promotes the removal of SUMO2/3 from KAP1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. Consequently, in the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. Thus, deSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair.

KW - homologous recombination

KW - HP1a

KW - KAP1

KW - SENP7

KW - SUMO

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JO - EMBO Reports

JF - EMBO Reports

IS - 11

ER -

The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair

Abstract

Keywords

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