The Humoral Immune Response to Asaccharolytic Eubacterium Species in Periodontitis

M. A. Slayne; W. G. Wade

The Humoral Immune Response to Asaccharolytic Eubacterium Species in Periodontitis

M. A. Slayne, W. G. Wade

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Abstract

Asaccharolytic Eubacterium species are strongly associated with advanced periodontal disease. Raised systemic antibody levels to Eubacterium brachy, E. nodutum and E. timidum have been found in periodontitis patients compared to healthy controls using ELISA and RIA. This study compared antibody profiles in periodontitis patients and controls against oral asaccharolytic Eubacterium species by Western blotting. Whole-cell proteins from strains of E. brachy, E. nodatum, E. timidum and representative strains of five candidate species were separated using PhastSystem SDS-PAGE. The proteins were electroblotted onto nitrocellulose and probed with 23 sera from periodontitis patients and 23 from periodontally healthy age- and sex-matched controls. Antibodies were present to proteins of all strains except E. nodatum but there was no relationship between patterns of antigen recognition and periodontal status

Original language	English
Pages (from-to)	283-286
Journal	MICROBIAL ECOLOGY IN HEALTH AND DISEASE
Volume	7
Publication status	Published - 1994

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title = "The Humoral Immune Response to Asaccharolytic Eubacterium Species in Periodontitis",

abstract = "Asaccharolytic Eubacterium species are strongly associated with advanced periodontal disease. Raised systemic antibody levels to Eubacterium brachy, E. nodutum and E. timidum have been found in periodontitis patients compared to healthy controls using ELISA and RIA. This study compared antibody profiles in periodontitis patients and controls against oral asaccharolytic Eubacterium species by Western blotting. Whole-cell proteins from strains of E. brachy, E. nodatum, E. timidum and representative strains of five candidate species were separated using PhastSystem SDS-PAGE. The proteins were electroblotted onto nitrocellulose and probed with 23 sera from periodontitis patients and 23 from periodontally healthy age- and sex-matched controls. Antibodies were present to proteins of all strains except E. nodatum but there was no relationship between patterns of antigen recognition and periodontal status",

author = "Slayne, {M. A.} and Wade, {W. G.}",

year = "1994",

language = "English",

volume = "7",

pages = "283--286",

journal = "MICROBIAL ECOLOGY IN HEALTH AND DISEASE",

issn = "0891-060X",

publisher = "Co-Action Publishing",

}

TY - JOUR

T1 - The Humoral Immune Response to Asaccharolytic Eubacterium Species in Periodontitis

AU - Slayne, M. A.

AU - Wade, W. G.

PY - 1994

Y1 - 1994

N2 - Asaccharolytic Eubacterium species are strongly associated with advanced periodontal disease. Raised systemic antibody levels to Eubacterium brachy, E. nodutum and E. timidum have been found in periodontitis patients compared to healthy controls using ELISA and RIA. This study compared antibody profiles in periodontitis patients and controls against oral asaccharolytic Eubacterium species by Western blotting. Whole-cell proteins from strains of E. brachy, E. nodatum, E. timidum and representative strains of five candidate species were separated using PhastSystem SDS-PAGE. The proteins were electroblotted onto nitrocellulose and probed with 23 sera from periodontitis patients and 23 from periodontally healthy age- and sex-matched controls. Antibodies were present to proteins of all strains except E. nodatum but there was no relationship between patterns of antigen recognition and periodontal status

AB - Asaccharolytic Eubacterium species are strongly associated with advanced periodontal disease. Raised systemic antibody levels to Eubacterium brachy, E. nodutum and E. timidum have been found in periodontitis patients compared to healthy controls using ELISA and RIA. This study compared antibody profiles in periodontitis patients and controls against oral asaccharolytic Eubacterium species by Western blotting. Whole-cell proteins from strains of E. brachy, E. nodatum, E. timidum and representative strains of five candidate species were separated using PhastSystem SDS-PAGE. The proteins were electroblotted onto nitrocellulose and probed with 23 sera from periodontitis patients and 23 from periodontally healthy age- and sex-matched controls. Antibodies were present to proteins of all strains except E. nodatum but there was no relationship between patterns of antigen recognition and periodontal status

M3 - Article

SN - 0891-060X

VL - 7

SP - 283

EP - 286

JO - MICROBIAL ECOLOGY IN HEALTH AND DISEASE

JF - MICROBIAL ECOLOGY IN HEALTH AND DISEASE

ER -

The Humoral Immune Response to Asaccharolytic Eubacterium Species in Periodontitis

Abstract

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