TY - JOUR
T1 - The influence of cholesterol, progesterone, 4-androstenedione and testosterone on the growth of Treponema denticola ATCC 33520 in batch cultures
AU - Clark, D T
AU - Soory, M
PY - 2006/10
Y1 - 2006/10
N2 - Previously, we have shown that reference and freshly isolated Treponema denticola cultures are capable of metabolising cholesterol, progesterone, 4-androstenedione and testosterone by means of 5 alpha-reductase, 3 beta-and 17 beta-hydroxysteroid dehydrogenase activity [Clark DT, Soory M. The metabolism of cholesterol and certain hormonal steroids by Treponema denticola. Steroids. 2006;71:352-63. [10]]. The aim of the work presented in this paper was to investigate the modulation of T denticola growth in batch cultures by these steroids, using T denticola ATCC 33520 as a model system. Growth curves were summarised using statistics based on optical density and protein yield. Cholesterol was found to stimulate growth at concentrations of 10 and 25 mu g/mL. Certain hormonal steroids inhibited the maximum achievable optical density at concentrations of 1 and 10 mu g/mL while the minimum concentration shown to inhibit protein yield was 0.001 mu g/mL of progesterone. The potential of the hormonal steroids to inhibit growth was in the order of progesterone, 4-androstenedione and testosterone. (c) 2006 Elsevier Ltd. All rights reserved
AB - Previously, we have shown that reference and freshly isolated Treponema denticola cultures are capable of metabolising cholesterol, progesterone, 4-androstenedione and testosterone by means of 5 alpha-reductase, 3 beta-and 17 beta-hydroxysteroid dehydrogenase activity [Clark DT, Soory M. The metabolism of cholesterol and certain hormonal steroids by Treponema denticola. Steroids. 2006;71:352-63. [10]]. The aim of the work presented in this paper was to investigate the modulation of T denticola growth in batch cultures by these steroids, using T denticola ATCC 33520 as a model system. Growth curves were summarised using statistics based on optical density and protein yield. Cholesterol was found to stimulate growth at concentrations of 10 and 25 mu g/mL. Certain hormonal steroids inhibited the maximum achievable optical density at concentrations of 1 and 10 mu g/mL while the minimum concentration shown to inhibit protein yield was 0.001 mu g/mL of progesterone. The potential of the hormonal steroids to inhibit growth was in the order of progesterone, 4-androstenedione and testosterone. (c) 2006 Elsevier Ltd. All rights reserved
U2 - 10.1016/j.anaerobe.2006.08.002
DO - 10.1016/j.anaerobe.2006.08.002
M3 - Article
VL - 12
SP - 267
EP - 273
JO - ANAEROBE
JF - ANAEROBE
IS - 5-6
ER -
