The Molecular Bases of the Dual Regulation of Bacterial Iron Sulfur Cluster Biogenesis by CyaY and IscX

Salvatore Adinolfi; Rita Puglisi; Jason C. Crack; Clara Iannuzzi; Fabrizio Dal Piaz; Petr V. Konarev; Dmitri I. Svergun; Stephen R. Martin; Nick E. Le Brun; Annalisa Pastore

doi:10.3389/fmolb.2017.00097

The Molecular Bases of the Dual Regulation of Bacterial Iron Sulfur Cluster Biogenesis by CyaY and IscX

Salvatore Adinolfi, Rita Puglisi, Jason C. Crack, Clara Iannuzzi, Fabrizio Dal Piaz, Petr V. Konarev, Dmitri I. Svergun, Stephen R. Martin, Nick E. Le Brun, Annalisa Pastore

Basic and Clinical Neuroscience

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

scX (or YfhJ) is a protein of unknown function which takes part in the iron-sulfur cluster assembly machinery, a highly specialized and essential metabolic pathway. IscX binds to iron with low affinity and interacts with IscS, the desulfurase central to cluster assembly.
Previous studies have suggested a competition between IscX and CyaY, the bacterial
ortholog of frataxin, for the same binding surface of IscS. This competition could suggest
a link between the two proteins with a functional significance. Using a hybrid approach based on nuclear magnetic resonance, small angle scattering and biochemical methods,
we show here that IscX is a modulator of the inhibitory properties of CyaY: by competing for the same site on IscS, the presence of IscX rescues the rates of enzymatic cluster formation which are inhibited by CyaY. The effect is stronger at low iron concentrations, whereas it becomes negligible at high iron concentrations. These results strongly suggest
the mechanism of the dual regulation of iron sulfur cluster assembly under the control of
iron as the effector.

Original language	English
Journal	Frontiers in Molecular Biosciences
DOIs	https://doi.org/10.3389/fmolb.2017.00097
Publication status	Published - 2 Feb 2018

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10.3389/fmolb.2017.00097Licence: CC BY

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@article{41155c5625af48cd96ab04168b13c2de,

title = "The Molecular Bases of the Dual Regulation of Bacterial Iron Sulfur Cluster Biogenesis by CyaY and IscX",

abstract = "scX (or YfhJ) is a protein of unknown function which takes part in the iron-sulfur cluster assembly machinery, a highly specialized and essential metabolic pathway. IscX binds to iron with low affinity and interacts with IscS, the desulfurase central to cluster assembly.Previous studies have suggested a competition between IscX and CyaY, the bacterialortholog of frataxin, for the same binding surface of IscS. This competition could suggesta link between the two proteins with a functional significance. Using a hybrid approach based on nuclear magnetic resonance, small angle scattering and biochemical methods,we show here that IscX is a modulator of the inhibitory properties of CyaY: by competing for the same site on IscS, the presence of IscX rescues the rates of enzymatic cluster formation which are inhibited by CyaY. The effect is stronger at low iron concentrations, whereas it becomes negligible at high iron concentrations. These results strongly suggestthe mechanism of the dual regulation of iron sulfur cluster assembly under the control ofiron as the effector.",

author = "Salvatore Adinolfi and Rita Puglisi and Crack, {Jason C.} and Clara Iannuzzi and {Dal Piaz}, Fabrizio and Konarev, {Petr V.} and Svergun, {Dmitri I.} and Martin, {Stephen R.} and {Le Brun}, {Nick E.} and Annalisa Pastore",

year = "2018",

month = feb,

day = "2",

doi = "10.3389/fmolb.2017.00097",

language = "English",

journal = "Frontiers in Molecular Biosciences",

issn = "2296-889X",

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T1 - The Molecular Bases of the Dual Regulation of Bacterial Iron Sulfur Cluster Biogenesis by CyaY and IscX

AU - Adinolfi, Salvatore

AU - Puglisi, Rita

AU - Crack, Jason C.

AU - Iannuzzi, Clara

AU - Dal Piaz, Fabrizio

AU - Konarev, Petr V.

AU - Svergun, Dmitri I.

AU - Martin, Stephen R.

AU - Le Brun, Nick E.

AU - Pastore, Annalisa

PY - 2018/2/2

Y1 - 2018/2/2

N2 - scX (or YfhJ) is a protein of unknown function which takes part in the iron-sulfur cluster assembly machinery, a highly specialized and essential metabolic pathway. IscX binds to iron with low affinity and interacts with IscS, the desulfurase central to cluster assembly.Previous studies have suggested a competition between IscX and CyaY, the bacterialortholog of frataxin, for the same binding surface of IscS. This competition could suggesta link between the two proteins with a functional significance. Using a hybrid approach based on nuclear magnetic resonance, small angle scattering and biochemical methods,we show here that IscX is a modulator of the inhibitory properties of CyaY: by competing for the same site on IscS, the presence of IscX rescues the rates of enzymatic cluster formation which are inhibited by CyaY. The effect is stronger at low iron concentrations, whereas it becomes negligible at high iron concentrations. These results strongly suggestthe mechanism of the dual regulation of iron sulfur cluster assembly under the control ofiron as the effector.

AB - scX (or YfhJ) is a protein of unknown function which takes part in the iron-sulfur cluster assembly machinery, a highly specialized and essential metabolic pathway. IscX binds to iron with low affinity and interacts with IscS, the desulfurase central to cluster assembly.Previous studies have suggested a competition between IscX and CyaY, the bacterialortholog of frataxin, for the same binding surface of IscS. This competition could suggesta link between the two proteins with a functional significance. Using a hybrid approach based on nuclear magnetic resonance, small angle scattering and biochemical methods,we show here that IscX is a modulator of the inhibitory properties of CyaY: by competing for the same site on IscS, the presence of IscX rescues the rates of enzymatic cluster formation which are inhibited by CyaY. The effect is stronger at low iron concentrations, whereas it becomes negligible at high iron concentrations. These results strongly suggestthe mechanism of the dual regulation of iron sulfur cluster assembly under the control ofiron as the effector.

U2 - 10.3389/fmolb.2017.00097

DO - 10.3389/fmolb.2017.00097

M3 - Article

SN - 2296-889X

JO - Frontiers in Molecular Biosciences

JF - Frontiers in Molecular Biosciences

ER -

The Molecular Bases of the Dual Regulation of Bacterial Iron Sulfur Cluster Biogenesis by CyaY and IscX

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