The role of CD8 T-cells in allergic lung inflammation

J Bienenstock; J Ring; A G Togias; D M Kemeny

The role of CD8 T-cells in allergic lung inflammation

J Bienenstock (Editor), J Ring (Editor), A G Togias (Editor), D M Kemeny

King's College London

Research output: Chapter in Book/Report/Conference proceeding › Conference paper

Abstract

We have established a murine T-cell transfer model to investigate the role of Tc2 cells in inflammatory lung diseases. CD8(+) T-cells were obtained from OVA-specific T-cell receptor transgenic C57BL/6 (OT-I) mice and subsequently used to generate Tc2 subset. Tc2 cells were adoptively transferred into naive C57BL/6 mice. Mice were then challenged daily with ovalbumin (OVA) aerosol on 3 consecutive days and the development of bronchial hyperresponsiveness (BHR) and eosinophilic inflammation measured. Transfer of Tc2 cells induced significant BHR and eosinophilia in OVA challenged mice as compared to the PBS controls. These results demonstrate that Tc2 cells in the lung can respond to inhaled soluble antigen and, therefore, may contribute to lung inflammation. Thus, the animal model used in this study will be a powerful tool ill understanding the role of Tc2 cells in allergic lung inflammation.

Original language	English
Title of host publication	Unknown
Place of Publication	TORONTO
Publisher	HOGREFE & HUBER PUBLISHERS
Pages	79 - 81
Number of pages	3
ISBN (Print)	0-88937-279-9
Publication status	Published - 2003
Event	27th Symposium of the Collegium-Internationale-Allergologicum - Southampton, Bermuda Duration: 1 Jan 2003 → …

Conference

Conference	27th Symposium of the Collegium-Internationale-Allergologicum
Country/Territory	Bermuda
City	Southampton
Period	1/01/2003 → …

Cite this

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title = "The role of CD8 T-cells in allergic lung inflammation",

abstract = "We have established a murine T-cell transfer model to investigate the role of Tc2 cells in inflammatory lung diseases. CD8(+) T-cells were obtained from OVA-specific T-cell receptor transgenic C57BL/6 (OT-I) mice and subsequently used to generate Tc2 subset. Tc2 cells were adoptively transferred into naive C57BL/6 mice. Mice were then challenged daily with ovalbumin (OVA) aerosol on 3 consecutive days and the development of bronchial hyperresponsiveness (BHR) and eosinophilic inflammation measured. Transfer of Tc2 cells induced significant BHR and eosinophilia in OVA challenged mice as compared to the PBS controls. These results demonstrate that Tc2 cells in the lung can respond to inhaled soluble antigen and, therefore, may contribute to lung inflammation. Thus, the animal model used in this study will be a powerful tool ill understanding the role of Tc2 cells in allergic lung inflammation.",

author = "J Bienenstock and J Ring and Togias, {A G} and Kemeny, {D M}",

year = "2003",

language = "English",

isbn = "0-88937-279-9",

pages = "79 -- 81",

booktitle = "Unknown",

publisher = "HOGREFE & HUBER PUBLISHERS",

note = "27th Symposium of the Collegium-Internationale-Allergologicum ; Conference date: 01-01-2003",

}

TY - CHAP

T1 - The role of CD8 T-cells in allergic lung inflammation

AU - Kemeny, D M

A2 - Bienenstock, J

A2 - Ring, J

A2 - Togias, A G

PY - 2003

Y1 - 2003

N2 - We have established a murine T-cell transfer model to investigate the role of Tc2 cells in inflammatory lung diseases. CD8(+) T-cells were obtained from OVA-specific T-cell receptor transgenic C57BL/6 (OT-I) mice and subsequently used to generate Tc2 subset. Tc2 cells were adoptively transferred into naive C57BL/6 mice. Mice were then challenged daily with ovalbumin (OVA) aerosol on 3 consecutive days and the development of bronchial hyperresponsiveness (BHR) and eosinophilic inflammation measured. Transfer of Tc2 cells induced significant BHR and eosinophilia in OVA challenged mice as compared to the PBS controls. These results demonstrate that Tc2 cells in the lung can respond to inhaled soluble antigen and, therefore, may contribute to lung inflammation. Thus, the animal model used in this study will be a powerful tool ill understanding the role of Tc2 cells in allergic lung inflammation.

AB - We have established a murine T-cell transfer model to investigate the role of Tc2 cells in inflammatory lung diseases. CD8(+) T-cells were obtained from OVA-specific T-cell receptor transgenic C57BL/6 (OT-I) mice and subsequently used to generate Tc2 subset. Tc2 cells were adoptively transferred into naive C57BL/6 mice. Mice were then challenged daily with ovalbumin (OVA) aerosol on 3 consecutive days and the development of bronchial hyperresponsiveness (BHR) and eosinophilic inflammation measured. Transfer of Tc2 cells induced significant BHR and eosinophilia in OVA challenged mice as compared to the PBS controls. These results demonstrate that Tc2 cells in the lung can respond to inhaled soluble antigen and, therefore, may contribute to lung inflammation. Thus, the animal model used in this study will be a powerful tool ill understanding the role of Tc2 cells in allergic lung inflammation.

M3 - Conference paper

SN - 0-88937-279-9

SP - 79

EP - 81

BT - Unknown

PB - HOGREFE & HUBER PUBLISHERS

CY - TORONTO

T2 - 27th Symposium of the Collegium-Internationale-Allergologicum

Y2 - 1 January 2003

ER -

The role of CD8 T-cells in allergic lung inflammation

Abstract

Conference

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