The use of mitochondrial cytochrome oxidase I gene (COI) to differentiate two UK blowfly species - Calliphora vicina and Calliphora vomitoria

Carole Ames; Bryan Turner; Barbara Daniel

doi:10.1016/j.forsciint.2006.01.005

The use of mitochondrial cytochrome oxidase I gene (COI) to differentiate two UK blowfly species - Calliphora vicina and Calliphora vomitoria

Carole Ames, Bryan Turner, Barbara Daniel

King's College London

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Traditionally identification of forensically important insects has been carried out based upon morphological differences between species. However insect evidence found at a crime scene may on occasion be difficult to distinguish by morphological techniques and under these circumstances another method of accurate identification is required. This work utilises a cytochrome oxidase I partial mitochondrial gene region (COI) to distinguish the two of the main UK blowfly species Calliphora vicina (Robineau Desvoidy) and Calliphora vomitoria (Linnaeus) (Diptera:Calliphoridae). Seventeen interspecific differences in COI sequence were located. Use of the restriction enzyme SfcI on this gene region provides a simple method for distinguishing between C. vicina and C. vomitoria. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	179-182
Number of pages	4
Journal	Forensic Science International
Volume	164
Issue number	2-3
DOIs	https://doi.org/10.1016/j.forsciint.2006.01.005
Publication status	Published - 20 Dec 2006

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title = "The use of mitochondrial cytochrome oxidase I gene (COI) to differentiate two UK blowfly species - Calliphora vicina and Calliphora vomitoria",

abstract = "Traditionally identification of forensically important insects has been carried out based upon morphological differences between species. However insect evidence found at a crime scene may on occasion be difficult to distinguish by morphological techniques and under these circumstances another method of accurate identification is required. This work utilises a cytochrome oxidase I partial mitochondrial gene region (COI) to distinguish the two of the main UK blowfly species Calliphora vicina (Robineau Desvoidy) and Calliphora vomitoria (Linnaeus) (Diptera:Calliphoridae). Seventeen interspecific differences in COI sequence were located. Use of the restriction enzyme SfcI on this gene region provides a simple method for distinguishing between C. vicina and C. vomitoria. (c) 2006 Elsevier Ireland Ltd. All rights reserved.",

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AU - Turner, Bryan

AU - Daniel, Barbara

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AB - Traditionally identification of forensically important insects has been carried out based upon morphological differences between species. However insect evidence found at a crime scene may on occasion be difficult to distinguish by morphological techniques and under these circumstances another method of accurate identification is required. This work utilises a cytochrome oxidase I partial mitochondrial gene region (COI) to distinguish the two of the main UK blowfly species Calliphora vicina (Robineau Desvoidy) and Calliphora vomitoria (Linnaeus) (Diptera:Calliphoridae). Seventeen interspecific differences in COI sequence were located. Use of the restriction enzyme SfcI on this gene region provides a simple method for distinguishing between C. vicina and C. vomitoria. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

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VL - 164

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JO - Forensic Science International

JF - Forensic Science International

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ER -

The use of mitochondrial cytochrome oxidase I gene (COI) to differentiate two UK blowfly species - Calliphora vicina and Calliphora vomitoria

Abstract

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