Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle

Fiona C. Lewis; Beverley J. Cottle; Victoria Shone; Giovanna Marazzi; David Sassoon; Cheyenne C.S. Tseng; Patricia Y.W. Dankers; Steven A.J. Chamuleau; Bernardo Nadal-Ginard; Georgina M. Ellison-Hughes

doi:10.1016/j.jacbts.2017.08.002

Transplantation of Allogeneic PW1^pos/Pax7^neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle

Fiona C. Lewis, Beverley J. Cottle, Victoria Shone, Giovanna Marazzi, David Sassoon, Cheyenne C.S. Tseng, Patricia Y.W. Dankers, Steven A.J. Chamuleau, Bernardo Nadal-Ginard, Georgina M. Ellison-Hughes

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Abstract

keletal muscle-derived PW1^pos/Pax7^neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.

Original language	English
Pages (from-to)	717-736
Journal	JACC: Basic to Translational Science
Volume	2
Issue number	6
Early online date	13 Dec 2017
DOIs	https://doi.org/10.1016/j.jacbts.2017.08.002
Publication status	Published - 2017

Keywords

allogeneic progenitor cells
growth factors
PICs
porcine preclinical model
regeneration
skeletal muscle

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10.1016/j.jacbts.2017.08.002Licence: CC BY-NC-ND

Transplantation of Allogeneic PW1pos/Pax7neg_Accepted16August2017_GOLD VoR (CC BY-NC-ND)Final published version, 4.17 MBLicence: CC BY-NC-ND

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Lewis, F. C., Cottle, B. J., Shone, V., Marazzi, G., Sassoon, D., Tseng, C. C. S., Dankers, P. Y. W., Chamuleau, S. A. J., Nadal-Ginard, B., & Ellison-Hughes, G. M. (2017). Transplantation of Allogeneic PW1^pos/Pax7^neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle. JACC: Basic to Translational Science, 2(6), 717-736. https://doi.org/10.1016/j.jacbts.2017.08.002

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title = "Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle",

abstract = "keletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.",

keywords = "allogeneic progenitor cells, growth factors, PICs, porcine preclinical model, regeneration, skeletal muscle",

author = "Lewis, {Fiona C.} and Cottle, {Beverley J.} and Victoria Shone and Giovanna Marazzi and David Sassoon and Tseng, {Cheyenne C.S.} and Dankers, {Patricia Y.W.} and Chamuleau, {Steven A.J.} and Bernardo Nadal-Ginard and Ellison-Hughes, {Georgina M.}",

year = "2017",

doi = "10.1016/j.jacbts.2017.08.002",

language = "English",

volume = "2",

pages = "717--736",

journal = "JACC: Basic to Translational Science",

issn = "2452-302X",

publisher = "Elsevier Inc.",

number = "6",

}

Lewis, FC , Cottle, BJ , Shone, V, Marazzi, G, Sassoon, D, Tseng, CCS, Dankers, PYW, Chamuleau, SAJ, Nadal-Ginard, B & Ellison-Hughes, GM 2017, 'Transplantation of Allogeneic PW1^pos/Pax7^neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle', JACC: Basic to Translational Science, vol. 2, no. 6, pp. 717-736. https://doi.org/10.1016/j.jacbts.2017.08.002

TY - JOUR

T1 - Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle

AU - Lewis, Fiona C.

AU - Cottle, Beverley J.

AU - Shone, Victoria

AU - Marazzi, Giovanna

AU - Sassoon, David

AU - Tseng, Cheyenne C.S.

AU - Dankers, Patricia Y.W.

AU - Chamuleau, Steven A.J.

AU - Nadal-Ginard, Bernardo

AU - Ellison-Hughes, Georgina M.

PY - 2017

Y1 - 2017

N2 - keletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.

AB - keletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.

KW - allogeneic progenitor cells

KW - growth factors

KW - PICs

KW - porcine preclinical model

KW - regeneration

KW - skeletal muscle

U2 - 10.1016/j.jacbts.2017.08.002

DO - 10.1016/j.jacbts.2017.08.002

M3 - Article

SN - 2452-302X

VL - 2

SP - 717

EP - 736

JO - JACC: Basic to Translational Science

JF - JACC: Basic to Translational Science

IS - 6

ER -

Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle

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Keywords

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Transplantation of Allogeneic PW1^pos/Pax7^neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle