Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages

Sílvia A Ferreira; Alexandra Correia; Pedro Madureira; Manuel Vilanova; Francisco M Gama

doi:10.1002/mabi.201200075

Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages

Sílvia A Ferreira, Alexandra Correia, Pedro Madureira, Manuel Vilanova, Francisco M Gama

Centre for Craniofacial & Regenerative Biology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.

Original language	English
Pages (from-to)	1172-80
Number of pages	9
Journal	MACROMOLECULAR BIOSCIENCE
Volume	12
Issue number	9
DOIs	https://doi.org/10.1002/mabi.201200075
Publication status	Published - Sept 2012

Keywords

Animals
Bone Marrow Cells
Drug Carriers
Endocytosis
Female
Gels
Macrophages
Mannans
Mice
Mice, Inbred BALB C
Nanostructures
Phagocytosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/mabi.201200075

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title = "Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages",

abstract = "The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.",

keywords = "Animals, Bone Marrow Cells, Drug Carriers, Endocytosis, Female, Gels, Macrophages, Mannans, Mice, Mice, Inbred BALB C, Nanostructures, Phagocytosis",

author = "Ferreira, {S{\'i}lvia A} and Alexandra Correia and Pedro Madureira and Manuel Vilanova and Gama, {Francisco M}",

note = "Copyright {\textcopyright} 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2012",

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doi = "10.1002/mabi.201200075",

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T1 - Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages

AU - Ferreira, Sílvia A

AU - Correia, Alexandra

AU - Madureira, Pedro

AU - Vilanova, Manuel

AU - Gama, Francisco M

PY - 2012/9

Y1 - 2012/9

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AB - The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.

KW - Animals

KW - Bone Marrow Cells

KW - Drug Carriers

KW - Endocytosis

KW - Female

KW - Gels

KW - Macrophages

KW - Mannans

KW - Mice

KW - Mice, Inbred BALB C

KW - Nanostructures

KW - Phagocytosis

U2 - 10.1002/mabi.201200075

DO - 10.1002/mabi.201200075

M3 - Article

C2 - 22807263

SN - 1616-5187

VL - 12

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JO - MACROMOLECULAR BIOSCIENCE

JF - MACROMOLECULAR BIOSCIENCE

IS - 9

ER -

Unraveling the uptake mechanisms of mannan nanogel in bone-marrow-derived macrophages

Abstract

Keywords

UN SDGs

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