@inbook{486282c22bd34e2cb512a275cc20a7fa,
title = "Use of Engineered Nanoparticles (ENPs) for the Study of High-Affinity IgE FcεRI Receptor Engagement and Rat Basophilic Leukemia (RBL) Cell Degranulation: Basophils and Mast Cells",
abstract = "Degranulation of mast cells and basophils occurs after the cross-linking of FcεRI receptor-bound IgE by multivalent allergens, resulting in the release of a range of de novo synthesized and preformed mediators of the allergic response. β-Hexosaminidase release is usually measured as a simple readout for degranulation. Furthermore, the rat basophilic leukemia (RBL)-2H3 cell line is commonly used for measuring degranulation, monitoring β-hexosaminidase release. Here, we describe surface-engineered and modified nanoparticles with specific ligands in order to study the signaling and cellular responses of the RBL-2H3 cell line.",
keywords = "Cross-linking, Degranulation, FcεRI, High-affinity receptor, IgE, Nanoparticles, Polystyrene, RBL-2H3",
author = "W Alelwani and Alharbi, {R. A.} and D Wan and Driton Vllasaliu and F Falcone and S Stolnik",
year = "2020",
month = aug,
day = "7",
doi = "10.1007/978-1-0716-0696-4_14",
language = "English",
isbn = "978-1-0716-0695-7",
volume = "2163",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "171--180",
booktitle = "Methods in Molecular Biology",
}