Video-rate dual-modal photoacoustic and fluorescence imaging through a multimode fibre towards forward-viewing endomicroscopy

Tianrui Zhao; Michelle Ma; Sebastien Ourselin; Tom Vercauteren; Wenfeng Xia

doi:10.1016/j.pacs.2021.100323

Video-rate dual-modal photoacoustic and fluorescence imaging through a multimode fibre towards forward-viewing endomicroscopy

Tianrui Zhao, Michelle Ma, Sebastien Ourselin, Tom Vercauteren, Wenfeng Xia

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Abstract

Multimode fibres (MMFs) are becoming increasingly attractive in optical endoscopy as they promise to enable unparallelled miniaturisation, spatial resolution and cost. However, high-speed imaging with wavefront shaping has been challenging. Here, we report the development of a video-rate dual-modal photoacoustic (PA) and fluorescence microscopy probe with a high-speed digital micromirror device (DMD) towards forward-viewing endomicroscopy. Optimal DMD patterns were obtained using a real-valued intensity transmission matrix algorithm to raster-scan a 1.5 μm-diameter focused beam at the distal fibre tip for imaging. The PA imaging speed and spatial resolution were varied from ∼2 to 57 frames per second and from 1.7 to 3 μm, respectively. Further, high-fidelity PA images of carbon fibres and mouse red blood cells were acquired at unprecedented speed. The capability of dual-modal imaging was demonstrated with phantoms. We anticipate that with further miniaturisation of the ultrasound detector, this probe could be integrated into medical needles to guide minimally invasive procedures.

Original language	English
Article number	100323
Number of pages	9
Journal	Photoacoustics
Volume	25
Early online date	31 Dec 2021
DOIs	https://doi.org/10.1016/j.pacs.2021.100323
Publication status	Published - Mar 2022

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1-s2.0-S2213597921000811-mainFinal published version, 2.39 MBLicence: CC BY

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title = "Video-rate dual-modal photoacoustic and fluorescence imaging through a multimode fibre towards forward-viewing endomicroscopy",

abstract = "Multimode fibres (MMFs) are becoming increasingly attractive in optical endoscopy as they promise to enable unparallelled miniaturisation, spatial resolution and cost. However, high-speed imaging with wavefront shaping has been challenging. Here, we report the development of a video-rate dual-modal photoacoustic (PA) and fluorescence microscopy probe with a high-speed digital micromirror device (DMD) towards forward-viewing endomicroscopy. Optimal DMD patterns were obtained using a real-valued intensity transmission matrix algorithm to raster-scan a 1.5 μm-diameter focused beam at the distal fibre tip for imaging. The PA imaging speed and spatial resolution were varied from ∼2 to 57 frames per second and from 1.7 to 3 μm, respectively. Further, high-fidelity PA images of carbon fibres and mouse red blood cells were acquired at unprecedented speed. The capability of dual-modal imaging was demonstrated with phantoms. We anticipate that with further miniaturisation of the ultrasound detector, this probe could be integrated into medical needles to guide minimally invasive procedures.",

author = "Tianrui Zhao and Michelle Ma and Sebastien Ourselin and Tom Vercauteren and Wenfeng Xia",

note = "Funding Information: This project was supported by the Academy of Medical Sciences/the Wellcome Trust/ the Government Department of Business, Energy and Industrial Strategy/the British Heart Foundation/Diabetes UK Springboard Award [ SBF006/1136 ], Wellcome Trust, United Kingdom ( 203148/Z/16/Z , WT101957 ) and Engineering and Physical Sciences Research Council, United Kingdom ( NS/A000027/1 , NS/A000049/1 ). We thank Dr Cinzia Imberti, Dr Charlotte Rivas and Dr Truc Pham from King{\textquoteright}s College London for helping with collecting mouse blood, and Mr. Keith Oakes from Elforlight Ltd. for technical support on the excitation laser. Publisher Copyright: {\textcopyright} 2021 The Authors",

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AU - Xia, Wenfeng

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N2 - Multimode fibres (MMFs) are becoming increasingly attractive in optical endoscopy as they promise to enable unparallelled miniaturisation, spatial resolution and cost. However, high-speed imaging with wavefront shaping has been challenging. Here, we report the development of a video-rate dual-modal photoacoustic (PA) and fluorescence microscopy probe with a high-speed digital micromirror device (DMD) towards forward-viewing endomicroscopy. Optimal DMD patterns were obtained using a real-valued intensity transmission matrix algorithm to raster-scan a 1.5 μm-diameter focused beam at the distal fibre tip for imaging. The PA imaging speed and spatial resolution were varied from ∼2 to 57 frames per second and from 1.7 to 3 μm, respectively. Further, high-fidelity PA images of carbon fibres and mouse red blood cells were acquired at unprecedented speed. The capability of dual-modal imaging was demonstrated with phantoms. We anticipate that with further miniaturisation of the ultrasound detector, this probe could be integrated into medical needles to guide minimally invasive procedures.

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Video-rate dual-modal photoacoustic and fluorescence imaging through a multimode fibre towards forward-viewing endomicroscopy

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