Zebrafish neural crest: Lessons and tools to study in vivo cell migration

Zain Alhashem, Macarena Alvarez Garcillan Portillo, Mint Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Härtel, Claudia Linker*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)

Abstract

The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages79-106
Number of pages28
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology
Volume2179
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell dissociation
  • Cell migration
  • Cell tracking
  • Clonal analysis
  • Gal4/Kalt4
  • In vivo imaging
  • Photoconversion
  • Tamoxifen
  • UAS
  • Zebrafish

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