Zebrafish promoter microarrays identify actively transcribed embryonic genes

Fiona C Wardle; Duncan T Odom; George W Bell; Bingbing Yuan; Timothy W Danford; Elisabeth L Wiellette; Elisabeth Herbolsheimer; Hazel L Sive; Richard A Young; James C Smith

doi:10.1016/j.semcdb.2005.11.008

Zebrafish promoter microarrays identify actively transcribed embryonic genes

Fiona C Wardle, Duncan T Odom, George W Bell, Bingbing Yuan, Timothy W Danford, Elisabeth L Wiellette, Elisabeth Herbolsheimer, Hazel L Sive, Richard A Young, James C Smith

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.

Original language	English
Pages (from-to)	R71
Number of pages	11
Journal	GENOME BIOLOGY
Volume	7
Issue number	8
DOIs	https://doi.org/10.1016/j.semcdb.2005.11.008
Publication status	Published - Feb 2006

Access to Document

10.1016/j.semcdb.2005.11.008

http://genomebiology.com/2006/7/8/R71

Cite this

@article{33190cae86f34470bff52d680c33194d,

title = "Zebrafish promoter microarrays identify actively transcribed embryonic genes",

abstract = "We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.",

author = "Wardle, {Fiona C} and Odom, {Duncan T} and Bell, {George W} and Bingbing Yuan and Danford, {Timothy W} and Wiellette, {Elisabeth L} and Elisabeth Herbolsheimer and Sive, {Hazel L} and Young, {Richard A} and Smith, {James C}",

year = "2006",

month = feb,

doi = "10.1016/j.semcdb.2005.11.008",

language = "English",

volume = "7",

pages = "R71",

journal = "GENOME BIOLOGY",

publisher = "BioMed Central",

number = "8",

}

TY - JOUR

T1 - Zebrafish promoter microarrays identify actively transcribed embryonic genes

AU - Wardle, Fiona C

AU - Odom, Duncan T

AU - Bell, George W

AU - Yuan, Bingbing

AU - Danford, Timothy W

AU - Wiellette, Elisabeth L

AU - Herbolsheimer, Elisabeth

AU - Sive, Hazel L

AU - Young, Richard A

AU - Smith, James C

PY - 2006/2

Y1 - 2006/2

N2 - We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.

AB - We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.

U2 - 10.1016/j.semcdb.2005.11.008

DO - 10.1016/j.semcdb.2005.11.008

M3 - Article

VL - 7

SP - R71

JO - GENOME BIOLOGY

JF - GENOME BIOLOGY

IS - 8

ER -

Zebrafish promoter microarrays identify actively transcribed embryonic genes

Abstract

Access to Document

Fingerprint

Cite this