Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems

Filippo Prischi, Annalisa Pastore*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Citations (Scopus)

Abstract

The current main challenge of Structural Biology is to undertake the structure determination of increasingly complex systems in the attempt to better understand their biological function. As systems become more challenging, however, there is an increasing demand for the parallel use of more than one independent technique to allow pushing the frontiers of structure determination and, at the same time, obtaining independent structural validation. The combination of different Structural Biology methods has been named hybrid approaches. The aim of this review is to critically discuss the most recent examples and new developments that have allowed structure determination or experimentally-based modelling of various molecular complexes selecting them among those that combine the use of nuclear magnetic resonance and small angle scattering techniques. We provide a selective but focused account of some of the most exciting recent approaches and discuss their possible further developments.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages351-368
Number of pages18
Volume896
ISBN (Electronic)9783319272160
ISBN (Print)9783319272146
DOIs
Publication statusPublished - 11 May 2016

Publication series

NameAdvances in Experimental Medicine and Biology
Volume896
ISSN (Print)00652598
ISSN (Electronic)22148019

Keywords

  • Multidomain proteins
  • NMR
  • Protein-protein interactions
  • SAXS
  • Structural biology

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