BDNF modulates sensory neuron synaptic activity by a facilitation of GABA transmission in the dorsal horn

S Pezet; J Cunningham; J Patel; J Grist; I Gavazzi; I J Lever; M Malcangio

doi:10.1006/mcne.2002.1166

BDNF modulates sensory neuron synaptic activity by a facilitation of GABA transmission in the dorsal horn

S Pezet, J Cunningham, J Patel, J Grist, I Gavazzi, I J Lever, M Malcangio

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

Topical application of brain-derived neurotrophic factor (BDNF) to the adult rat isolated dorsal horn with dorsal root attached preparation inhibited the electrically evoked release of substance P (SP) from sensory neurons. This effect of BDNF was dose dependent (EC50 250 pM) and reversed by the tyrosine kinase inhibitor, K-252a. BDNF-induced inhibition of SP release was blocked by the GABA(B) receptor antagonist CGP 55485 but not by naloxone. Acute application of BDNF significantly increased potassium-stimulated release of GABA in the dorsal horn isolated in vitro and this effect was blocked by K-252a. Intrathecal injection of BDNF into the rat lumbar spinal cord induced a short-lasting increase in hindpaw threshold to noxious thermal stimulation that was blocked by CGP 55485 and was associated with activation of ERK in dorsal horn. These data suggest that exogenous BDNF can indirectly modulate primary sensory neuron synaptic efficacy via facilitation of the release of GABA from dorsal horn interneurons.

Original language	English
Pages (from-to)	51 - 62
Number of pages	12
Journal	Molecular and Cellular Neurosciences
Volume	21
Issue number	1
DOIs	https://doi.org/10.1006/mcne.2002.1166
Publication status	Published - 2002

Access to Document

10.1006/mcne.2002.1166

Cite this

@article{1eddc81306714fe0867caada17015a22,

title = "BDNF modulates sensory neuron synaptic activity by a facilitation of GABA transmission in the dorsal horn",

abstract = "Topical application of brain-derived neurotrophic factor (BDNF) to the adult rat isolated dorsal horn with dorsal root attached preparation inhibited the electrically evoked release of substance P (SP) from sensory neurons. This effect of BDNF was dose dependent (EC50 250 pM) and reversed by the tyrosine kinase inhibitor, K-252a. BDNF-induced inhibition of SP release was blocked by the GABA(B) receptor antagonist CGP 55485 but not by naloxone. Acute application of BDNF significantly increased potassium-stimulated release of GABA in the dorsal horn isolated in vitro and this effect was blocked by K-252a. Intrathecal injection of BDNF into the rat lumbar spinal cord induced a short-lasting increase in hindpaw threshold to noxious thermal stimulation that was blocked by CGP 55485 and was associated with activation of ERK in dorsal horn. These data suggest that exogenous BDNF can indirectly modulate primary sensory neuron synaptic efficacy via facilitation of the release of GABA from dorsal horn interneurons.",

author = "S Pezet and J Cunningham and J Patel and J Grist and I Gavazzi and Lever, {I J} and M Malcangio",

year = "2002",

doi = "10.1006/mcne.2002.1166",

language = "English",

volume = "21",

pages = "51 -- 62",

journal = "Molecular and Cellular Neurosciences",

publisher = "ACADEMIC PRESS INC",

number = "1",

}

TY - JOUR

T1 - BDNF modulates sensory neuron synaptic activity by a facilitation of GABA transmission in the dorsal horn

AU - Pezet, S

AU - Cunningham, J

AU - Patel, J

AU - Grist, J

AU - Gavazzi, I

AU - Lever, I J

AU - Malcangio, M

PY - 2002

Y1 - 2002

N2 - Topical application of brain-derived neurotrophic factor (BDNF) to the adult rat isolated dorsal horn with dorsal root attached preparation inhibited the electrically evoked release of substance P (SP) from sensory neurons. This effect of BDNF was dose dependent (EC50 250 pM) and reversed by the tyrosine kinase inhibitor, K-252a. BDNF-induced inhibition of SP release was blocked by the GABA(B) receptor antagonist CGP 55485 but not by naloxone. Acute application of BDNF significantly increased potassium-stimulated release of GABA in the dorsal horn isolated in vitro and this effect was blocked by K-252a. Intrathecal injection of BDNF into the rat lumbar spinal cord induced a short-lasting increase in hindpaw threshold to noxious thermal stimulation that was blocked by CGP 55485 and was associated with activation of ERK in dorsal horn. These data suggest that exogenous BDNF can indirectly modulate primary sensory neuron synaptic efficacy via facilitation of the release of GABA from dorsal horn interneurons.

AB - Topical application of brain-derived neurotrophic factor (BDNF) to the adult rat isolated dorsal horn with dorsal root attached preparation inhibited the electrically evoked release of substance P (SP) from sensory neurons. This effect of BDNF was dose dependent (EC50 250 pM) and reversed by the tyrosine kinase inhibitor, K-252a. BDNF-induced inhibition of SP release was blocked by the GABA(B) receptor antagonist CGP 55485 but not by naloxone. Acute application of BDNF significantly increased potassium-stimulated release of GABA in the dorsal horn isolated in vitro and this effect was blocked by K-252a. Intrathecal injection of BDNF into the rat lumbar spinal cord induced a short-lasting increase in hindpaw threshold to noxious thermal stimulation that was blocked by CGP 55485 and was associated with activation of ERK in dorsal horn. These data suggest that exogenous BDNF can indirectly modulate primary sensory neuron synaptic efficacy via facilitation of the release of GABA from dorsal horn interneurons.

UR - http://www.scopus.com/inward/record.url?scp=0036396622&partnerID=8YFLogxK

U2 - 10.1006/mcne.2002.1166

DO - 10.1006/mcne.2002.1166

M3 - Article

VL - 21

SP - 51

EP - 62

JO - Molecular and Cellular Neurosciences

JF - Molecular and Cellular Neurosciences

IS - 1

ER -

BDNF modulates sensory neuron synaptic activity by a facilitation of GABA transmission in the dorsal horn

Abstract

Access to Document

Other files and links

Fingerprint

Cite this