Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

Abhinav Kumar; Elif Melis Bicer; Anna Babin Morgan; Paul Pfeffer; Marco Monopoli; Kenneth A Dawson; Jonny Eriksson; Katarina Edwards; Steven Lynham; Matthew Arno; Annelie F Behndig; Anders Blomberg; Graham Somers; Dave Hassall; Lea Ann Dailey; Ben Forbes; Ian S Mudway

doi:10.1016/j.nano.2015.12.369

Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

Abhinav Kumar, Elif Melis Bicer, Anna Babin Morgan, Paul Pfeffer, Marco Monopoli, Kenneth A Dawson, Jonny Eriksson, Katarina Edwards, Steven Lynham, Matthew Arno, Annelie F Behndig, Anders Blomberg, Graham Somers, Dave Hassall, Lea Ann Dailey, Ben Forbes, Ian S Mudway

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate quantitative profiles of the proteins within the corona formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.

Original language	English
Pages (from-to)	1033–1043
Journal	Nanomedicine-Nanotechnology Biology And Medicine
Volume	12
Issue number	4
Early online date	6 Jan 2016
DOIs	https://doi.org/10.1016/j.nano.2015.12.369
Publication status	Published - 1 May 2016

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Kumar, A., Bicer, E. M., Morgan, A. B., Pfeffer, P., Monopoli, M., Dawson, K. A., Eriksson, J., Edwards, K., Lynham, S., Arno, M., Behndig, A. F., Blomberg, A., Somers, G., Hassall, D., Dailey, L. A., Forbes, B., & Mudway, I. S. (2016). Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid. Nanomedicine-Nanotechnology Biology And Medicine, 12(4), 1033–1043. https://doi.org/10.1016/j.nano.2015.12.369

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title = "Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid",

abstract = "When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate quantitative profiles of the proteins within the corona formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.",

author = "Abhinav Kumar and Bicer, {Elif Melis} and Morgan, {Anna Babin} and Paul Pfeffer and Marco Monopoli and Dawson, {Kenneth A} and Jonny Eriksson and Katarina Edwards and Steven Lynham and Matthew Arno and Behndig, {Annelie F} and Anders Blomberg and Graham Somers and Dave Hassall and Dailey, {Lea Ann} and Ben Forbes and Mudway, {Ian S}",

note = "Copyright {\textcopyright} 2015. Published by Elsevier Inc.",

year = "2016",

month = may,

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doi = "10.1016/j.nano.2015.12.369",

language = "English",

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Kumar, A , Bicer, EM , Morgan, AB , Pfeffer, P, Monopoli, M, Dawson, KA, Eriksson, J, Edwards, K, Lynham, S , Arno, M, Behndig, AF, Blomberg, A, Somers, G, Hassall, D, Dailey, LA , Forbes, B & Mudway, IS 2016, 'Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid', Nanomedicine-Nanotechnology Biology And Medicine, vol. 12, no. 4, pp. 1033–1043. https://doi.org/10.1016/j.nano.2015.12.369

TY - JOUR

T1 - Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

AU - Kumar, Abhinav

AU - Bicer, Elif Melis

AU - Morgan, Anna Babin

AU - Pfeffer, Paul

AU - Monopoli, Marco

AU - Dawson, Kenneth A

AU - Eriksson, Jonny

AU - Edwards, Katarina

AU - Lynham, Steven

AU - Arno, Matthew

AU - Behndig, Annelie F

AU - Blomberg, Anders

AU - Somers, Graham

AU - Hassall, Dave

AU - Dailey, Lea Ann

AU - Forbes, Ben

AU - Mudway, Ian S

PY - 2016/5/1

Y1 - 2016/5/1

N2 - When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate quantitative profiles of the proteins within the corona formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.

AB - When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate quantitative profiles of the proteins within the corona formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.

U2 - 10.1016/j.nano.2015.12.369

DO - 10.1016/j.nano.2015.12.369

M3 - Article

C2 - 26767511

SN - 1549-9634

VL - 12

SP - 1033

EP - 1043

JO - Nanomedicine-Nanotechnology Biology And Medicine

JF - Nanomedicine-Nanotechnology Biology And Medicine

IS - 4

ER -

Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

Abstract

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