Evidence for A Macromolecular Complex in Poor Prognostic CLL That Contains CD38, CD49d, CD44, MMP-9 and ZAP-70

Andrea Buggins; Ana Levi; Satyen Gohill; Keith Fishlock; Piers Patten; Deborah Yallop; Ghulam Mufti; Stephen Devereux

Evidence for A Macromolecular Complex in Poor Prognostic CLL That Contains CD38, CD49d, CD44, MMP-9 and ZAP-70

Andrea Buggins, Ana Levi, Satyen Gohill, Keith Fishlock, Piers Patten, Deborah Yallop, Ghulam Mufti, Stephen Devereux

King's College London

Research output: Contribution to journal › Meeting abstract

Abstract

The outcome for patients with Chronic Lymphocytic Leukemia (CLL) is highly variable and correlates with clinical parameters such as disease stage, as well as various biological markers that reflect the genomic, phenotypic and functional properties of the tumor and its microenvironment. CD38, Cd49d and ZAP-70 are three such markers that are associated with a poor clinical outcome and which have previously been shown to play a role in migration. Progressive disease is invariably associated with tissue invasion. In the present study we investigated the possibility that the markers CD38, CD49d and ZAP-70 might be physically associated as well as functionally linked. Using 4-color flow cytometry we measured the level of expression of CD38 and CD49d by CD19+/CD5+ CLL cells in patients with CLL presenting to our routine diagnostic laboratory. A correlation was observed between both the percentage positivity and mean fluorescent intensity of CD38 and CD49d expression (r2=0.42, p=

Original language	English
Article number	2334
Journal	Blood
Publication status	Published - 2009
Event	American Society of Hematology - New Orleans Duration: 4 Dec 2009 → 8 Dec 2009

Cite this

@article{a77bb3d07f964ff2a0a74679d466e1b8,

title = "Evidence for A Macromolecular Complex in Poor Prognostic CLL That Contains CD38, CD49d, CD44, MMP-9 and ZAP-70",

abstract = "The outcome for patients with Chronic Lymphocytic Leukemia (CLL) is highly variable and correlates with clinical parameters such as disease stage, as well as various biological markers that reflect the genomic, phenotypic and functional properties of the tumor and its microenvironment. CD38, Cd49d and ZAP-70 are three such markers that are associated with a poor clinical outcome and which have previously been shown to play a role in migration. Progressive disease is invariably associated with tissue invasion. In the present study we investigated the possibility that the markers CD38, CD49d and ZAP-70 might be physically associated as well as functionally linked. Using 4-color flow cytometry we measured the level of expression of CD38 and CD49d by CD19+/CD5+ CLL cells in patients with CLL presenting to our routine diagnostic laboratory. A correlation was observed between both the percentage positivity and mean fluorescent intensity of CD38 and CD49d expression (r2=0.42, p=",

author = "Andrea Buggins and Ana Levi and Satyen Gohill and Keith Fishlock and Piers Patten and Deborah Yallop and Ghulam Mufti and Stephen Devereux",

year = "2009",

language = "English",

journal = "Blood",

issn = "1528-0020",

publisher = "American Society of Hematology",

note = "American Society of Hematology ; Conference date: 04-12-2009 Through 08-12-2009",

}

TY - JOUR

T1 - Evidence for A Macromolecular Complex in Poor Prognostic CLL That Contains CD38, CD49d, CD44, MMP-9 and ZAP-70

AU - Buggins, Andrea

AU - Levi, Ana

AU - Gohill, Satyen

AU - Fishlock, Keith

AU - Patten, Piers

AU - Yallop, Deborah

AU - Mufti, Ghulam

AU - Devereux, Stephen

PY - 2009

Y1 - 2009

N2 - The outcome for patients with Chronic Lymphocytic Leukemia (CLL) is highly variable and correlates with clinical parameters such as disease stage, as well as various biological markers that reflect the genomic, phenotypic and functional properties of the tumor and its microenvironment. CD38, Cd49d and ZAP-70 are three such markers that are associated with a poor clinical outcome and which have previously been shown to play a role in migration. Progressive disease is invariably associated with tissue invasion. In the present study we investigated the possibility that the markers CD38, CD49d and ZAP-70 might be physically associated as well as functionally linked. Using 4-color flow cytometry we measured the level of expression of CD38 and CD49d by CD19+/CD5+ CLL cells in patients with CLL presenting to our routine diagnostic laboratory. A correlation was observed between both the percentage positivity and mean fluorescent intensity of CD38 and CD49d expression (r2=0.42, p=

AB - The outcome for patients with Chronic Lymphocytic Leukemia (CLL) is highly variable and correlates with clinical parameters such as disease stage, as well as various biological markers that reflect the genomic, phenotypic and functional properties of the tumor and its microenvironment. CD38, Cd49d and ZAP-70 are three such markers that are associated with a poor clinical outcome and which have previously been shown to play a role in migration. Progressive disease is invariably associated with tissue invasion. In the present study we investigated the possibility that the markers CD38, CD49d and ZAP-70 might be physically associated as well as functionally linked. Using 4-color flow cytometry we measured the level of expression of CD38 and CD49d by CD19+/CD5+ CLL cells in patients with CLL presenting to our routine diagnostic laboratory. A correlation was observed between both the percentage positivity and mean fluorescent intensity of CD38 and CD49d expression (r2=0.42, p=

M3 - Meeting abstract

SN - 1528-0020

JO - Blood

JF - Blood

M1 - 2334

T2 - American Society of Hematology

Y2 - 4 December 2009 through 8 December 2009

ER -

Evidence for A Macromolecular Complex in Poor Prognostic CLL That Contains CD38, CD49d, CD44, MMP-9 and ZAP-70

Abstract

Fingerprint

Cite this