@inbook{6a28d8ead2b6473c9bda11d4c34d438f,
title = "FLIPR Calcium Mobilization Assays in GPCR Drug Discovery",
abstract = "Intracellular calcium mobilization can be measured using several methods varying in indicator dyes and devices used. In this chapter, we describe the fluorescence-based method (FLIPR Calcium 4 Assay) developed by Molecular Devices for a FlexStation and routinely used in our laboratory for detecting intracellular calcium changes. The assay is designed to study calcium mobilization induced by majority of GPCRs and calcium channels and allows for simultaneous concentration-dependent analysis of several receptor agonists and antagonists, useful in receptor characterization and drug discovery projects.",
keywords = "Airway smooth muscle cells, Calcium mobilization, FlexStation, Fluorescence, G-protein-coupled receptors, Mast cells",
author = "Grzegorz Woszczek and Elisabeth Fuerst and Maguire, {Thomas J.A.}",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
doi = "10.1007/978-1-0716-1221-7_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc",
pages = "193--205",
booktitle = "Methods in Molecular Biology",
}