Harvesting biomolecules from tissue by porous silicon nanoneedles

Davide A. Martella*, Ciro Chiappini

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference paperpeer-review

Abstract

Precision oncology is increasingly focusing on alternative strategies to tissue biopsy, for less invasive collection of biological samples containing optimal information for molecular diagnostics. Ordered arrays of porous-silicon nanoneedles (pSi-nNs) has the potential to harvest RNA, proteins and lipids from with lower invasiveness than tissue biopsy. We characterized the fabrication of nNs, to perform fine-tuning of their porosity and shape through metal-assisted chemical etching (MACE) and reactive ion etching (RIE). We interfaced nNs with tissue to evaluate the total yield of harvested biomolecules, finding an amount of RNA, proteins and lipids comparable with that extracted from a 30μm tissue section.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1087-1088
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 4 Oct 20209 Oct 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period4/10/20209/10/2020

Keywords

  • Molecular Diagnostics
  • Nanoneedles
  • Porous Silicon

Fingerprint

Dive into the research topics of 'Harvesting biomolecules from tissue by porous silicon nanoneedles'. Together they form a unique fingerprint.

Cite this