@article{1422f60925494dcbaa791dd4e802a689,
title = "Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics",
abstract = "The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022).",
keywords = "Immunology, Microbiology, Molecular Biology",
author = "Robert Page and Edward Scourfield and Mattia Ficarelli and McKellar, {Stuart W.} and Lee, {Kwok Leung} and Maguire, {Thomas J.A.} and Clement Bouton and Lista, {Maria Jose} and Neil, {Stuart J.D.} and Malim, {Michael H.} and Mark Zuckerman and Mischo, {Hannah E.} and Martinez-Nunez, {Rocio T.}",
note = "Funding Information: This work was funded by a King's College London King{\textquoteright}s Together Rapid COVID-19 Call award to R.T.M.-N. and S.J.D.N. and a Huo Family Foundation award to M.H.M., R.T.M.-N., and S.J.D.N. H.E.M. and S.W.M. were funded by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society ( 218537/Z/19/Z ) to H.E.M. R.T.M.-N. was supported by the Wellcome Trust ( 213984/Z/18/Z ). R.P. was supported through the NIHR Biomedical Research Centre based at Guy{\textquoteright}s and St. Thomas{\textquoteright} NHS Foundation Trust in partnership with King{\textquoteright}s College London and King{\textquoteright}s College Hospital NHS Foundation Trust. M.F. was supported by the MRC -KCL Doctoral Training Partnership in Biomedical Sciences ( MR/N013700/1 ) and MRC ( MR/R50225X/1 ). This research was funded in whole, or in part, by the Wellcome Trust 218537/Z/19/Z and 213984/Z/18/Z . For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. Funding Information: This work was funded by a King's College London King's Together Rapid COVID-19 Call award to R.T.M.-N. and S.J.D.N. and a Huo Family Foundation award to M.H.M. R.T.M.-N. and S.J.D.N. H.E.M. and S.W.M. were funded by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (218537/Z/19/Z) to H.E.M. R.T.M.-N. was supported by the Wellcome Trust (213984/Z/18/Z). R.P. was supported through the NIHR Biomedical Research Centre based at Guy's and St. Thomas{\textquoteright} NHS Foundation Trust in partnership with King's College London and King's College Hospital NHS Foundation Trust. M.F. was supported by the MRC-KCL Doctoral Training Partnership in Biomedical Sciences (MR/N013700/1) and MRC (MR/R50225X/1). This research was funded in whole, or in part, by the Wellcome Trust 218537/Z/19/Z and 213984/Z/18/Z. For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. R.P. E.S. M.F. S.W.M. K.L.L. T.J.A.M. C.B. and M.J.L. performed experiments. S.J.D.N. M.H.M. and M.Z. provided intellectual input and samples. H.E.M. and R.T.M.-N. supervised the project and designed and performed experiments. R.P. H.E.M. and R.T.M.-N. wrote the initial manuscript. All authors were involved in editing the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = jun,
day = "17",
doi = "10.1016/j.xpro.2022.101300",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}