TY - JOUR
T1 - In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy
AU - Høgset, Håkon
AU - Horgan, Conor C.
AU - Armstrong, James P.K.
AU - Bergholt, Mads S.
AU - Torraca, Vincenzo
AU - Chen, Qu
AU - Keane, Timothy J.
AU - Bugeon, Laurence
AU - Dallman, Margaret J.
AU - Mostowy, Serge
AU - Stevens, Molly M.
N1 - Funding Information:
H.H. acknowledges funding from Aker Scholarship. C.C.H. acknowledges funding from the NanoMed Marie Skłodowska-Curie ITN from the H2020 programme under grant number 676137. M.S.B. acknowledges support from H2020 through the Individual Marie Skłodowska-Curie Fellowship “IMAGINE” (701713). J.P.K.A. acknowledges support from Arthritis Research U.K. (21138) and the Medical Research Council (MR/S00551X/ 1). Q.C. and M.M.S. acknowledge support from the European Research Council (ERC) Seventh Framework Programme Consolidator grant “Naturale CG” under grant agreement no. 616417. T.J.K. acknowledges the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Individual European Fellowship (Grant Agreement No. 746980). M.M.S. acknowledges Wellcome Trust Senior Investigator Award (098411/Z/12/Z). Research in the Mostowy laboratory is supported by a European Research Council Consolidator Grant (772853 - ENTRAPMENT), Wellcome Trust Senior Research Fellowship (206444/Z/17/Z), and the Lister Institute of Preventive Medicine. V.T. acknowledges support from H2020 through the Individual Marie Skłodowska-Curie Fellowship “MYCO TRAPS” (700088). The authors thank John Goertz for help with statistical analysis, Brittany Rae for help with zebrafish husbandry, Lorraine Lawrence for preparation of histological sections, Vernon LaLone and Ralf Wenz for input during the manuscript preparation.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12
Y1 - 2020/12
N2 - Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.
AB - Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.
UR - http://www.scopus.com/inward/record.url?scp=85097019344&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-19827-1
DO - 10.1038/s41467-020-19827-1
M3 - Article
C2 - 33268772
AN - SCOPUS:85097019344
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 6172
ER -