Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction

Simon D. Goldenberg; Maria Gumban; Anthony Hall; Amita Patel; Gary L. French

doi:10.1016/j.diagmicrobio.2011.03.012

Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction

Simon D. Goldenberg, Maria Gumban, Anthony Hall, Amita Patel, Gary L. French

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Abstract

Glutamate dehydrogenase (GDH) is popular as a preliminary test for the detection of Clostridium difficile. Recent work has suggested that GDH sensitivity may vary according to ribotype and may be lower for ribotypes 002, 027, and 106 compared with polymerase chain reaction (PCR). We investigated this effect using a dilution series of 64 isolates tested by GDH and Cepheid GeneXpert PCR. PCR was significantly more sensitive than GDH overall; however, there was no difference in detection according to specific ribotype. Crown Copyright (c) 2011 Published by Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	417 - 419
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2011.03.012
Publication status	Published - Jul 2011

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10.1016/j.diagmicrobio.2011.03.012

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title = "Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction",

abstract = "Glutamate dehydrogenase (GDH) is popular as a preliminary test for the detection of Clostridium difficile. Recent work has suggested that GDH sensitivity may vary according to ribotype and may be lower for ribotypes 002, 027, and 106 compared with polymerase chain reaction (PCR). We investigated this effect using a dilution series of 64 isolates tested by GDH and Cepheid GeneXpert PCR. PCR was significantly more sensitive than GDH overall; however, there was no difference in detection according to specific ribotype. Crown Copyright (c) 2011 Published by Elsevier Inc. All rights reserved.",

author = "Goldenberg, {Simon D.} and Maria Gumban and Anthony Hall and Amita Patel and French, {Gary L.}",

year = "2011",

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T1 - Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction

AU - Goldenberg, Simon D.

AU - Gumban, Maria

AU - Hall, Anthony

AU - Patel, Amita

AU - French, Gary L.

PY - 2011/7

Y1 - 2011/7

N2 - Glutamate dehydrogenase (GDH) is popular as a preliminary test for the detection of Clostridium difficile. Recent work has suggested that GDH sensitivity may vary according to ribotype and may be lower for ribotypes 002, 027, and 106 compared with polymerase chain reaction (PCR). We investigated this effect using a dilution series of 64 isolates tested by GDH and Cepheid GeneXpert PCR. PCR was significantly more sensitive than GDH overall; however, there was no difference in detection according to specific ribotype. Crown Copyright (c) 2011 Published by Elsevier Inc. All rights reserved.

AB - Glutamate dehydrogenase (GDH) is popular as a preliminary test for the detection of Clostridium difficile. Recent work has suggested that GDH sensitivity may vary according to ribotype and may be lower for ribotypes 002, 027, and 106 compared with polymerase chain reaction (PCR). We investigated this effect using a dilution series of 64 isolates tested by GDH and Cepheid GeneXpert PCR. PCR was significantly more sensitive than GDH overall; however, there was no difference in detection according to specific ribotype. Crown Copyright (c) 2011 Published by Elsevier Inc. All rights reserved.

U2 - 10.1016/j.diagmicrobio.2011.03.012

DO - 10.1016/j.diagmicrobio.2011.03.012

M3 - Article

VL - 70

SP - 417

EP - 419

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

Lack of effect of strain type on detection of toxigenic Clostridium difficile by glutamate dehydrogenase and polymerase chain reaction

Abstract

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