@article{9539ccd373164f16824be08a77b639e6,
title = "Shigella Serotypes Associated With Carriage in Humans Establish Persistent Infection in Zebrafish",
abstract = "Shigella represents a paraphyletic group of enteroinvasive Escherichia coli. More than 40 Shigella serotypes have been reported. However, most cases within the men who have sex with men (MSM) community are attributed to 3 serotypes: Shigella sonnei unique serotype and Shigella flexneri 2a and 3a serotypes. Using the zebrafish model, we demonstrate that Shigella can establish persistent infection in vivo. Bacteria are not cleared by the immune system and become antibiotic tolerant. Establishment of persistent infection depends on the O-antigen, a key constituent of the bacterial surface and a serotype determinant. Representative isolates associated with MSM transmission persist in zebrafish, while representative isolates of a serotype not associated with MSM transmission do not. Isolates of a Shigella serotype establishing persistent infections elicited significantly less macrophage death in vivo than isolates of a serotype unable to persist. We conclude that zebrafish are a valuable platform to illuminate factors underlying establishment of Shigella persistent infection in humans.",
keywords = "Humans, Male, Animals, Zebrafish, Serogroup, Homosexuality, Male, Persistent Infection, Sexual and Gender Minorities, Shigella, Dysentery, Bacillary/microbiology, Shigella flexneri",
author = "Vincenzo Torraca and Dominik Brokatzky and Miles, {Sydney L.} and Chong, {Charlotte E.} and {De Silva}, {P. Malaka} and Stephen Baker and Claire Jenkins and Holt, {Kathryn E.} and Baker, {Kate S.} and Serge Mostowy",
note = "Funding Information: Financial support. V. T. was supported by a London School of Hygiene and Tropical Medicine/Wellcome Trust Institutional Strategic Support Fund fellowship (204928/Z/16/Z). S. L. M. is supported by a PhD studentship (BB/T008709/1) from the London Interdisciplinary Doctoral Programme, Biotechnology and Biological Sciences Research Council. D. B. is supported by the Deutsche Forschungsgemeinschaft Walter Benjamin Programme (BR 6637/1-1). Research in the laboratory of K. S. B. is supported by an Academy of Medical Sciences Springboard award (SBF002\1114), a Medical Research Council New Investigator award (MR/R020787/1), and the Biotechnology and Biological Sciences Research Council (BB/V009184/1). Research in the laboratory of S. M. is supported by a European Research Council Consolidator Grant (772853; ENTRAPMENT), Wellcome Trust Senior Research Fellowship (206444/Z/17/Z), and the Lister Institute of Preventive Medicine. Funding to pay the Open Access publication charges for this article was provided by the London School of Hygiene and Tropical Medicine. Publisher Copyright: {\textcopyright} 2023 The Author(s). Published by Oxford University Press on behalf of Infectious Diseases Society of America.",
year = "2023",
month = oct,
day = "15",
doi = "10.1093/infdis/jiad326",
language = "English",
volume = "228",
pages = "1108--1118",
journal = "The Journal of infectious diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "8",
}
