Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Yuan Zhao; Mohamed  Uduman; Jacqueline Siu; Thomas Justin Tull; Jeremy Sanderson; Yu-Chang Bryan Wu; Julian Q Zhou; Nedyalko Petrov; Richard Jonathan Ellis; Sarah Katrina Todd; Konstantia-Maria Chavele; William Wilfried Jonathan Guesdon; Anna Vossenkamper; Wayel Jassem; David D’Cruz; David Jonathan Fear; Susan John; dagmar Scheel-Toellner; claire hopkins; Estefania  Moreno; Natalie  Woodman; Francesca Donatella Ciccarelli; Susanne Heck; Steven Kleinstein; Mats Bemark; Jo Michele Spencer

doi:10.1038/s41467-018-06089-1

Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Yuan Zhao, Mohamed Uduman, Jacqueline Siu, Thomas Justin Tull, Jeremy Sanderson, Yu-Chang Bryan Wu, Julian Q Zhou, Nedyalko Petrov, Richard Jonathan Ellis, Sarah Katrina Todd, Konstantia-Maria Chavele, William Wilfried Jonathan Guesdon, Anna Vossenkamper, Wayel Jassem, David D’Cruz, David Jonathan Fear, Susan John, dagmar Scheel-Toellner, claire hopkins, Estefania MorenoNatalie Woodman, Francesca Donatella Ciccarelli, Susanne Heck, Steven Kleinstein, Mats Bemark, Jo Michele Spencer

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

352 Downloads (Pure)

Abstract

Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC where somatic mutations are acquired.

Original language	English
Article number	3857
Number of pages	15
Journal	Nature Communications
Volume	9
Issue number	1
Early online date	21 Sept 2018
DOIs	https://doi.org/10.1038/s41467-018-06089-1
Publication status	Published - 21 Sept 2018

Access to Document

10.1038/s41467-018-06089-1Licence: CC BY

Spatiotemporal segregation of human_ZHAO_Published21September2018_GOLD CORRECTED PROOF (CC BY)Proof, 6.94 MBLicence: CC BY

Cite this

Zhao, Y., Uduman, M., Siu, J., Tull, T. J., Sanderson, J., Wu, Y.-C. B., Zhou, J. Q., Petrov, N., Ellis, R. J., Todd, S. K., Chavele, K.-M., Guesdon, W. W. J., Vossenkamper, A., Jassem, W., D’Cruz, D., Fear, D. J., John, S., Scheel-Toellner, D., hopkins, C., ... Spencer, J. M. (2018). Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue. Nature Communications, 9(1), Article 3857. https://doi.org/10.1038/s41467-018-06089-1

@article{5af81ed2d3c646e9aa67b82c739e5c3c,

title = "Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue",

abstract = "Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature na{\"i}ve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC where somatic mutations are acquired.",

author = "Yuan Zhao and Mohamed Uduman and Jacqueline Siu and Tull, {Thomas Justin} and Jeremy Sanderson and Wu, {Yu-Chang Bryan} and Zhou, {Julian Q} and Nedyalko Petrov and Ellis, {Richard Jonathan} and Todd, {Sarah Katrina} and Konstantia-Maria Chavele and Guesdon, {William Wilfried Jonathan} and Anna Vossenkamper and Wayel Jassem and David D{\textquoteright}Cruz and Fear, {David Jonathan} and Susan John and dagmar Scheel-Toellner and claire hopkins and Estefania Moreno and Natalie Woodman and Ciccarelli, {Francesca Donatella} and Susanne Heck and Steven Kleinstein and Mats Bemark and Spencer, {Jo Michele}",

year = "2018",

month = sep,

day = "21",

doi = "10.1038/s41467-018-06089-1",

language = "English",

volume = "9",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

number = "1",

}

Zhao, Y, Uduman, M, Siu, J, Tull, TJ , Sanderson, J , Wu, Y-CB, Zhou, JQ, Petrov, N, Ellis, RJ , Todd, SK , Chavele, K-M , Guesdon, WWJ, Vossenkamper, A, Jassem, W, D’Cruz, D , Fear, DJ , John, S, Scheel-Toellner, D, hopkins, C, Moreno, E, Woodman, N, Ciccarelli, FD, Heck, S, Kleinstein, S, Bemark, M & Spencer, JM 2018, 'Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue', Nature Communications, vol. 9, no. 1, 3857. https://doi.org/10.1038/s41467-018-06089-1

TY - JOUR

T1 - Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

AU - Zhao, Yuan

AU - Uduman, Mohamed

AU - Siu, Jacqueline

AU - Tull, Thomas Justin

AU - Sanderson, Jeremy

AU - Wu, Yu-Chang Bryan

AU - Zhou, Julian Q

AU - Petrov, Nedyalko

AU - Ellis, Richard Jonathan

AU - Todd, Sarah Katrina

AU - Chavele, Konstantia-Maria

AU - Guesdon, William Wilfried Jonathan

AU - Vossenkamper, Anna

AU - Jassem, Wayel

AU - D’Cruz, David

AU - Fear, David Jonathan

AU - John, Susan

AU - Scheel-Toellner, dagmar

AU - hopkins, claire

AU - Moreno, Estefania

AU - Woodman, Natalie

AU - Ciccarelli, Francesca Donatella

AU - Heck, Susanne

AU - Kleinstein, Steven

AU - Bemark, Mats

AU - Spencer, Jo Michele

PY - 2018/9/21

Y1 - 2018/9/21

N2 - Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC where somatic mutations are acquired.

AB - Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27-CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organized gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC where somatic mutations are acquired.

UR - http://www.scopus.com/inward/record.url?scp=85053755692&partnerID=8YFLogxK

U2 - 10.1038/s41467-018-06089-1

DO - 10.1038/s41467-018-06089-1

M3 - Article

SN - 2041-1723

VL - 9

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 3857

ER -

Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Abstract

Access to Document

Other files and links

Fingerprint

Cite this