Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy

Uchechukwu Agwuegbo; Kim Carol Jonas

doi:10.1016/bs.mcb.2021.12.001

Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy

Uchechukwu Agwuegbo, Kim Carol Jonas^*

^*Corresponding author for this work

Women & Children's Health

Research output: Contribution to journal › Article › peer-review

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Abstract

G protein-coupled receptor (GPCR) di/oligomerization has revealed potential mechanisms for receptors diversification of signal selectivity, specificity, and amplitude. The use of super-resolution imaging techniques to investigate these di/oligomer molecular complexities have undoubtably provided insight to the dynamics of complexes formed at the plasma membrane. Here we describe the methodology of photoactivatable dye localization microscopy (PD-PALM) to study the spatial organization of GPCR homomers at the plasma membrane.

Original language	English
Pages (from-to)	27-41
Number of pages	15
Journal	Methods in Cell Biology
Early online date	29 Jan 2022
DOIs	https://doi.org/10.1016/bs.mcb.2021.12.001
Publication status	Published - Jan 2022

Keywords

Dimerization
G protein-coupled receptor
Oligomerization
PD-PALM
Single-molecule imaging

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10.1016/bs.mcb.2021.12.001

Agwuegbo and Jonas_acceptedLicence: Unspecified

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title = "Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy",

abstract = "G protein-coupled receptor (GPCR) di/oligomerization has revealed potential mechanisms for receptors diversification of signal selectivity, specificity, and amplitude. The use of super-resolution imaging techniques to investigate these di/oligomer molecular complexities have undoubtably provided insight to the dynamics of complexes formed at the plasma membrane. Here we describe the methodology of photoactivatable dye localization microscopy (PD-PALM) to study the spatial organization of GPCR homomers at the plasma membrane.",

keywords = "Dimerization, G protein-coupled receptor, Oligomerization, PD-PALM, Single-molecule imaging",

author = "Uchechukwu Agwuegbo and Jonas, {Kim Carol}",

note = "Funding Information: This work was supported by National Institutes of Health (P01AG029531), BBSRC grant (BB/R015961/1 and BB/R015961/2), and Society for Reproduction and Fertility Covid-19 support scheme for PhD Students 2021. We thank the Facility for Imaging of Light Microscopy at Imperial College London for technical support in PD-PALM imaging. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

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doi = "10.1016/bs.mcb.2021.12.001",

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journal = "Methods in Cell Biology",

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N2 - G protein-coupled receptor (GPCR) di/oligomerization has revealed potential mechanisms for receptors diversification of signal selectivity, specificity, and amplitude. The use of super-resolution imaging techniques to investigate these di/oligomer molecular complexities have undoubtably provided insight to the dynamics of complexes formed at the plasma membrane. Here we describe the methodology of photoactivatable dye localization microscopy (PD-PALM) to study the spatial organization of GPCR homomers at the plasma membrane.

AB - G protein-coupled receptor (GPCR) di/oligomerization has revealed potential mechanisms for receptors diversification of signal selectivity, specificity, and amplitude. The use of super-resolution imaging techniques to investigate these di/oligomer molecular complexities have undoubtably provided insight to the dynamics of complexes formed at the plasma membrane. Here we describe the methodology of photoactivatable dye localization microscopy (PD-PALM) to study the spatial organization of GPCR homomers at the plasma membrane.

KW - Dimerization

KW - G protein-coupled receptor

KW - Oligomerization

KW - PD-PALM

KW - Single-molecule imaging

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M3 - Article

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SN - 0091-679X

SP - 27

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JO - Methods in Cell Biology

JF - Methods in Cell Biology

ER -

Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy

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Keywords

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